Sterile Fecal Microbiota Transplantation Boosts Anti-Inflammatory T-Cell Response in Ulcerative Colitis Patients
Full article
Journal |
International Journal of Molecular Sciences
, E-ISSN: 1422-0067
|
Output data |
Year: 2024,
Volume: 25,
Number: 3,
Article number
: 1886,
Pages count
:
DOI:
10.3390/ijms25031886
|
Tags |
ulcerative colitis; sterile fecal microbiota transplantation; regulatory T cells; immune response |
Authors |
Chechushkov Anton
1,2
,
Desyukevich Pavel
2
,
Yakovlev Timur
2
,
Al Allaf Lina
2
,
Shrainer Evgeniya
3,2
,
Morozov Vitalyi
2
,
Tikunova Nina
4,2
|
Affiliations |
1 |
Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia
|
2 |
Federal State Public Scientific Institution “Institute of Chemical Biology and Fundamental Medicine”, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia
|
3 |
Autonomous Non-Commercial Organization “Center of New Medical Technologies in Akademgorodok”, 630090 Novosibirsk, Russia
|
4 |
Advanced Engineering School, Novosibirsk State University, 630090 Novosibirsk, Russia
|
|
Funding (2)
1
|
МИНИСТЕРСТВО НАУКИ И ВЫСШЕГО ОБРАЗОВАНИЯ РОССИЙСКОЙ ФЕДЕРАЦИИ
|
ПФНИ РФ (2021-2030) 0245-2021-0008
|
2
|
Russian Science Foundation
|
РНФ № 21-14-00360
|
Ulcerative colitis is a chronic immune-mediated disease of unclear etiology, affecting people of different ages and significantly reducing the quality of life. Modern methods of therapy are mainly represented by anti-inflammatory drugs and are not aimed at a specific pathogenetic factor. In this study, we investigated the effect of transplantation of sterile stool filtrate from healthy donors on the induction of anti-inflammatory immune mechanisms. It was shown that performing such a procedure in patients with ulcerative colitis caused the appearance of T helper cells in the blood, which reacted to the content of sterile stool filtrates in an antigen-specific manner and produced IL-10. At the same time, cells of the same patients before therapy in response to the addition of sterile stool filtrates were less reactive and predominantly produced IL-4, indicating its pro-inflammatory skewing. The obtained data demonstrated the effect of an anti-inflammatory shift in the T-helper response after transplantation of sterile stool filtrate, which increased and persisted for at least three months after the procedure.