Preservation of mechanical and morphological properties of porcine cardiac outflow vessels after decellularization and long-term wet storage

Preservation of mechanical and morphological properties of porcine cardiac outflow vessels after decellularization and long-term wet storage Full article

Journal

Biomimetics
ISSN: 23137673

Output data

Year: 2023, Volume: 8, Number: 3, Article number : 315, Pages count : DOI: 10.3390/biomimetics8030315

Tags

bioprosthetic valve; decellularization; tissue storage; uniaxial mechanical testing; tissue engineering; extracellular matrix

Authors

Sergeevichev D. ^1,2 , Vasiliyeva M. ³ , Kuznetsova E. ¹ , Chelobanov B. ⁴

Affiliations

1	Meshalkin National Medical Research Center
2	N.N. Vorozhtsov Institute of Organic Chemistry, SB RAS
3	Novosibirsk State University
4	Institute of Chemical Biology and Fundamental Medicine SB RAS

: Widely used storage methods, including freezing or chemical modification, preserve the sterility of biological tissues but degrade the mechanical properties of materials used to make heart valve prostheses. Therefore, wet storage remains the most optimal option for biomaterials. Three biocidal solutions (an antibiotic mixture, an octanediol-phenoxyethanol complex solution, and a glycerol-ethanol mixture) were studied for the storage of native and decellularized porcine aorta and pulmonary trunk. Subsequent mechanical testing and microstructural analysis showed a slight increase in the tensile strength of native and decellularized aorta in the longitudinal direction. Pulmonary trunk elongation increased 1.3–1.6 times in the longitudinal direction after decellularization only. The microstructures of the tested specimens showed no differences before and after wet storage. Thus, two months of wet storage of native and decellularized porcine aorta and pulmonary trunks does not significantly affect the strength and elastic properties of the material. The wet storage protocol using alcohol solutions of glycerol or octanediol-phenoxyethanol mixture may be intended for further fabrication of extracellular matrix for tissue-engineered biological heart valve prostheses.

Sergeevichev D. , Vasiliyeva M. , Kuznetsova E. , Chelobanov B.
Preservation of mechanical and morphological properties of porcine cardiac outflow vessels after decellularization and long-term wet storage
Biomimetics. 2023. V.8. N3. 315 . DOI: 10.3390/biomimetics8030315 WOS Scopus РИНЦ OpenAlex

Published print:

Jul 15, 2023

Web of science:	WOS:001035076100001
Scopus:	2-s2.0-85166308467
Elibrary:	54959467
OpenAlex:	W4384698811

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