Preservation of mechanical and morphological properties of porcine cardiac outflow vessels after decellularization and long-term wet storage Full article
| Journal |
Biomimetics
ISSN: 23137673 |
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| Output data | Year: 2023, Volume: 8, Number: 3, Article number : 315, Pages count : DOI: 10.3390/biomimetics8030315 | ||||||||
| Tags | bioprosthetic valve; decellularization; tissue storage; uniaxial mechanical testing; tissue engineering; extracellular matrix | ||||||||
| Authors |
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| Affiliations |
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Abstract:
: Widely used storage methods, including freezing or chemical modification, preserve the
sterility of biological tissues but degrade the mechanical properties of materials used to make heart
valve prostheses. Therefore, wet storage remains the most optimal option for biomaterials. Three
biocidal solutions (an antibiotic mixture, an octanediol-phenoxyethanol complex solution, and a
glycerol-ethanol mixture) were studied for the storage of native and decellularized porcine aorta
and pulmonary trunk. Subsequent mechanical testing and microstructural analysis showed a slight
increase in the tensile strength of native and decellularized aorta in the longitudinal direction. Pulmonary trunk elongation increased 1.3–1.6 times in the longitudinal direction after decellularization
only. The microstructures of the tested specimens showed no differences before and after wet storage.
Thus, two months of wet storage of native and decellularized porcine aorta and pulmonary trunks
does not significantly affect the strength and elastic properties of the material. The wet storage
protocol using alcohol solutions of glycerol or octanediol-phenoxyethanol mixture may be intended
for further fabrication of extracellular matrix for tissue-engineered biological heart valve prostheses.
Cite:
Sergeevichev D.
, Vasiliyeva M.
, Kuznetsova E.
, Chelobanov B.
Preservation of mechanical and morphological properties of porcine cardiac outflow vessels after decellularization and long-term wet storage
Biomimetics. 2023. V.8. N3. 315 . DOI: 10.3390/biomimetics8030315 WOS Scopus РИНЦ OpenAlex
Preservation of mechanical and morphological properties of porcine cardiac outflow vessels after decellularization and long-term wet storage
Biomimetics. 2023. V.8. N3. 315 . DOI: 10.3390/biomimetics8030315 WOS Scopus РИНЦ OpenAlex
Dates:
| Published print: | Jul 15, 2023 |
Identifiers:
| Web of science: | WOS:001035076100001 |
| Scopus: | 2-s2.0-85166308467 |
| Elibrary: | 54959467 |
| OpenAlex: | W4384698811 |