Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering Full article
| Journal |
Fermentation
ISSN: 2311-5637 |
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| Output data | Year: 2023, Volume: 9, Number: 10, Article number : 874, Pages count : DOI: 10.3390/fermentation9100874 | ||||
| Tags | DNA cleavage; phosphodiester bond hydrolysis; restriction endonuclease; restriction– modification; protein-DNA interaction; structural family; catalytic mechanism; kinetics | ||||
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| Affiliations |
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Funding (1)
| 1 | МИНИСТЕРСТВО НАУКИ И ВЫСШЕГО ОБРАЗОВАНИЯ РОССИЙСКОЙ ФЕДЕРАЦИИ | 2021-1930-ФП5-8365-8981 |
Abstract:
: Restriction endonucleases are a component of restriction–modification systems, where the
main biological function is to protect bacterial cells from incoming foreign DNA molecules. There are
four main types of restriction enzymes (types I, II, III, and IV), which differ in protein composition,
cofactor requirements, and mode of action. The most studied are representatives of type II, which
specifically recognize DNA sequences of 4–8 bp and catalyze DNA cleavage within these sequences
or not far from them. The exceptional precision of type II enzymes has made them indispensable for
DNA manipulations. Although hundreds of DNA restriction enzymes are currently known, there
is still a need for enzymes that recognize new DNA targets. For this reason, the discovery of new
natural restriction endonucleases and rational design of their properties (to obtain enzymes with
high specificity for a unique nucleotide sequence at a restriction site and without nonspecific activity)
will expand the list of enzymes for use in biotechnology and genetic engineering. This review briefly
touches upon the main types of restriction endonucleases, their classification, nomenclature, and typical
properties, and it concisely describes approaches to the construction of enzymes with altered properties
Cite:
Alekseeva I.V.
, Kuznetsov N.A.
Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering
Fermentation. 2023. V.9. N10. 874 . DOI: 10.3390/fermentation9100874 WOS Scopus OpenAlex
Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering
Fermentation. 2023. V.9. N10. 874 . DOI: 10.3390/fermentation9100874 WOS Scopus OpenAlex
Dates:
| Published print: | Sep 28, 2023 |
Identifiers:
| Web of science: | WOS:001089851600001 |
| Scopus: | 2-s2.0-85175091730 |
| OpenAlex: | W4387140516 |