Influence of the Composition of Cationic Liposomes on the Performance of Cargo Immunostimulatory RNA Научная публикация
| Журнал |
Pharmaceutics
ISSN: 1999-4923 |
||||
|---|---|---|---|---|---|
| Вых. Данные | Год: 2023, Том: 15, Номер статьи : 2184, Страниц : DOI: 10.3390/pharmaceutics15092184 | ||||
| Ключевые слова | immunostimulatory RNA; cationic liposomes; lipoconjugates; cytokine-inducing activity; antitumor activity; IFN alpha | ||||
| Авторы |
|
||||
| Организации |
|
Реферат:
: In this study, the impact of different delivery systems on the cytokine-inducing, antiproliferative, and antitumor activities of short immunostimulatory double-stranded RNA (isRNA) was
investigated. The delivery systems, consisting of the polycationic amphiphile 1,26-bis(cholest-5-en-3-
yloxycarbonylamino)-7,11,16,20 tetraazahexacosan tetrahydrochloride (2X3), and the lipid-helper
dioleoylphosphatidylethanolamine (DOPE), were equipped with polyethylene glycol lipoconjugates differing in molecular weight and structure. The main findings of this work are as follows:
(i) significant activation of MCP-1 and INF-α, β, and γ production in CBA mice occurs under the
action of isRNA complexes with liposomes containing lipoconjugates with long PEG chains, while activation of MCP-1 and INF-γ, but not INF-α or β, was observed under the action of isRNA lipoplexes
containing lipoconjugates with short PEG chains; (ii) a pronounced antiproliferative effect on B16
melanoma cells in vitro, as well as an antitumor and hepatoprotective effect in vivo, was induced by
isRNA pre-complexes with non-pegylated liposomes, while complexes containing lipoconjugates
with long-chain liposomes were inactive; (iii) the antitumor activity of isRNA correlated with the
efficiency of its accumulation in the cells and did not explicitly depend on the activation of cytokine
and interferon production. Thus, the structure of the delivery system plays a vital role in determining
the response to isRNA and allows for the choice of a delivery system depending on the desired effect.
Библиографическая ссылка:
Bishani А.
, Makarova D.M.
, Shmendel E.V.
, Maslov M.A.
, Sen'kova A.V.
, Savin I.A.
, Gladkikh D.V.
, Zenkova M.A.
, Chernolovskaya E.L.
Influence of the Composition of Cationic Liposomes on the Performance of Cargo Immunostimulatory RNA
Pharmaceutics. 2023. V.15. 2184 . DOI: 10.3390/pharmaceutics15092184 WOS Scopus PMID OpenAlex
Influence of the Composition of Cationic Liposomes on the Performance of Cargo Immunostimulatory RNA
Pharmaceutics. 2023. V.15. 2184 . DOI: 10.3390/pharmaceutics15092184 WOS Scopus PMID OpenAlex
Даты:
| Опубликована в печати: | 23 авг. 2023 г. |
Идентификаторы БД:
| Web of science: | WOS:001072244100001 |
| Scopus: | 2-s2.0-85172453730 |
| PMID (PubMed): | 37765155 |
| OpenAlex: | W4386098950 |