Effects of Dendrimer-microRNA Nanoformulations against Glioblastoma Stem Cells

Effects of Dendrimer-microRNA Nanoformulations against Glioblastoma Stem Cells Full article

Journal

Pharmaceutics
ISSN: 1999-4923

Output data

Year: 2023, Volume: 15, Number: 3, Pages: 968-968 Pages count : 1 DOI: 10.3390/pharmaceutics15030968

Tags

3D tumor models; dendrimers; glioblastoma; microRNA; nanomedicine; nucleic acid therapeutics; surface markers; tumor stem cells

Authors

Knauer Nadezhda ^1,2 , Meschaninova Mariya I. ³ , Muhammad Sajjad ² , Hänggi Daniel ² , Majoral Jean‐Pierre ⁴ , Kahlert Ulf D. ⁵ , Kozlov V.А. ¹ , Apartsin Evgeny ⁶

Affiliations

1	Clinic for Neurosurgery, Medical Faculty, Heinrich-Heine University Medical Center Düsseldorf, 40225 Düsseldorf, Germany
2	Research Institute of Fundamental and Clinical Immunology, 630099 Novosibirsk, Russia
3	Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, Russia
4	Laboratoire de Chimie de Coordination, CNRS, 205 Route de Narbonne, CEDEX 04, 31077 Toulouse, France
5	Molecular and Experimental Surgery, Clinic for General-, Visceral-, Vascular-, and Transplant-Surgery, Medical Faculty, University Hospital Magdeburg, 39120 Magdeburg, Germany
6	Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, 33600 Pessac, France

Glioblastoma is a rapidly progressing tumor quite resistant to conventional treatment. These features are currently assigned to a self-sustaining population of glioblastoma stem cells. Anti-tumor stem cell therapy calls for a new means of treatment. In particular, microRNA-based treatment is a solution, which in turn requires specific carriers for intracellular delivery of functional oligonucleotides. Herein, we report a preclinical in vitro validation of antitumor activity of nanoformulations containing antitumor microRNA miR-34a and microRNA-21 synthetic inhibitor and polycationic phosphorus and carbosilane dendrimers. The testing was carried out in a panel of glioblastoma and glioma cell lines, glioblastoma stem-like cells and induced pluripotent stem cells. We have shown dendrimer-microRNA nanoformulations to induce cell death in a controllable manner, with cytotoxic effects being more pronounced in tumor cells than in non-tumor stem cells. Furthermore, nanoformulations affected the expression of proteins responsible for interactions between the tumor and its immune microenvironment: surface markers (PD-L1, TIM3, CD47) and IL-10. Our findings evidence the potential of dendrimer-based therapeutic constructions for the anti-tumor stem cell therapy worth further investigation.

Knauer N. , Meschaninova M.I. , Muhammad S. , Hänggi D. , Majoral J. , Kahlert U.D. , Kozlov V.А. , Apartsin E.
Effects of Dendrimer-microRNA Nanoformulations against Glioblastoma Stem Cells
Pharmaceutics. 2023. V.15. N3. P.968-968. DOI: 10.3390/pharmaceutics15030968 WOS Scopus OpenAlex

Published print:

Mar 17, 2023

Web of science:	WOS:000957822200001
Scopus:	2-s2.0-85151733480
OpenAlex:	W4327730487

DB	Citing
OpenAlex	5
Scopus	5
Web of science	3