Complexes and Supramolecular Associates of Dodecyl-Containing Oligonucleotides with Serum Albumin Full article
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Biochemistry (Moscow)
ISSN: 0006-2979 |
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| Output data | Year: 2023, Volume: 88, Number: 8, Pages: 1165-1180 Pages count : 16 DOI: 10.1134/S0006297923080102 | ||
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Abstract:
Serum albumin is currently in the focus of biomedical research as a promising platform for the creation of multi-component self-assembling systems due to the presence of several sites with high binding affinity of various compounds in its molecule, including lipophilic oligonucleotide conjugates. In this work, we investigated the stoichiometry of the dodecyl-containing oligonucleotides binding to bovine and human serum albumins using an electrophoretic mobility shift assay. The results indicate the formation of the albumin-oligonucleotide complexes with a stoichiometry of about 1 : (1.25 ± 0.25) under physiological-like conditions. Using atomic force microscopy, it was found that the interaction of human serum albumin with the duplex of complementary dodecyl-containing oligonucleotides resulted in the formation of circular associates with a diameter of 165.5 ± 94.3 nm and 28.9 ± 16.9 nm in height, and interaction with polydeoxyadenylic acid and dodecyl-containing oligothymidylate resulted in formation of supramolecular associates with the size of about 315.4 ± 70.9 and 188.3 ± 43.7 nm, respectively. The obtained data allow considering the dodecyl-containing oligonucle-otides and albumin as potential components of the designed self-assembling systems for solving problems of molecular biology, biomedicine, and development of unique theranostics with targeted action.
Cite:
Pavlova A.S.
, Iliusenko V.V.
, Kupryushkin M.S.
, Zharkov T.D.
, Dyudeeva E.S.
, Bauer I.A.
, Chubarov A.S.
, Pyshnyi D.V.
, Pyshnaya I.A.
Complexes and Supramolecular Associates of Dodecyl-Containing Oligonucleotides with Serum Albumin
Biochemistry (Moscow). 2023. V.88. N8. P.1165-1180. DOI: 10.1134/S0006297923080102 WOS Scopus PMID OpenAlex
Complexes and Supramolecular Associates of Dodecyl-Containing Oligonucleotides with Serum Albumin
Biochemistry (Moscow). 2023. V.88. N8. P.1165-1180. DOI: 10.1134/S0006297923080102 WOS Scopus PMID OpenAlex
Original:
Павлова А.С.
, Илющенко В.В.
, Купрюшкин М.С.
, Жарков Т.Д.
, Дюдеева Е.С.
, Бауэр И.А.
, Чубаров А.С.
, Пышный Д.В.
, Пышная И.А.
Комплексы и высокомолекулярные ассоциаты додецил-содержащих олигонуклеотидов с сывороточным альбумином
БИОХИМИЯ. 2023. Т.88. №8. С.1423 – 1440. DOI: 10.31857/S0320972523080109 РИНЦ OpenAlex
Комплексы и высокомолекулярные ассоциаты додецил-содержащих олигонуклеотидов с сывороточным альбумином
БИОХИМИЯ. 2023. Т.88. №8. С.1423 – 1440. DOI: 10.31857/S0320972523080109 РИНЦ OpenAlex
Identifiers:
| Web of science: | WOS:001050483000010 |
| Scopus: | 2-s2.0-85168475198 |
| PMID: | 37758315 |
| OpenAlex: | W4385955838 |