DNA Probes for Analysis of the Activity of Key Enzymes of the Base Excision DNA Repair Pathway in Human Cells

DNA Probes for Analysis of the Activity of Key Enzymes of the Base Excision DNA Repair Pathway in Human Cells Full article

Journal

Molecular Biology
ISSN: 0026-8933

Output data

Year: 2023, Volume: 57, Number: 2, Pages: 299-311 Pages count : 13 DOI: 10.1134/s0026893323020024

Authors

Alekseeva I.V. ¹ , Kuznetsova A.A. ¹ , Kladova O.A. ¹ , Shender V.O. ² , Schneider P.V. ² , Fedorova O.S. ¹ , Kuznetsov N.A. ^1,3

Affiliations

1	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia
2	Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia
3	Novosibirsk National Research State University, Novosibirsk, Russia

Funding (2)

1	МИНИСТЕРСТВО НАУКИ И ВЫСШЕГО ОБРАЗОВАНИЯ РОССИЙСКОЙ ФЕДЕРАЦИИ	ПФНИ РФ (2021-2030) 0245-2021-0009
2	Russian Science Foundation	РНФ №21-14-00018

The important role of DNA damage in the occurrence of various diseases, including cancer, has led to study of the mechanisms of genetic information stability, that have been carried out since the discovery of DNA repair systems. The question of the relationship between the accumulation of DNA damage, disorders in DNA repair pathways, and increased risk of disease development is still relevant. Over the past few years, significant efforts have been made to develop methods for analyzing the activity of DNA repair enzymes in human cells. In this work, we developed fluorescent DNA probes that allow us to determine the activity of key enzymes of base excision DNA repair in cell extracts, namely the DNA glycosylases UNG2, SMUG1, MBD4, TDG, AAG, NEIL1, NTHL1, and OGG1 and the AP endonuclease APE1. The sensitivity of DNA probes was determined on pure enzyme preparations. Determination of the activity of repair enzymes in cell extracts of the human ovarian tumor lines TOV112, 79, OVCAR3, MESOV, SCOV3, and TOV21 revealed significant variability in the level of enzyme activity in these cell lines. These results may become a test system platform for analyzing the activity of the base excision DNA repair system in the human body.

Alekseeva I.V. , Kuznetsova A.A. , Kladova O.A. , Shender V.O. , Schneider P.V. , Fedorova O.S. , Kuznetsov N.A.
DNA Probes for Analysis of the Activity of Key Enzymes of the Base Excision DNA Repair Pathway in Human Cells
Molecular Biology. 2023. V.57. N2. P.299-311. DOI: 10.1134/s0026893323020024 WOS Scopus OpenAlex

Алексеева И.В. , Кузнецова А.А. , Кладова О.А. , Шендер В.О. , Шнайдер П.В. , Федорова О.С. , Кузнецов Н.А.
Разработка и апробация ДНК-зондов для определения активности ключевых ферментов пути эксцизионной репарации оснований ДНК в клетках человека
Молекулярная биология. 2023. Т.57. №2. С.316-329. DOI: 10.31857/S0026898423020027 РИНЦ OpenAlex

Web of science:	WOS:000984409600015
Scopus:	2-s2.0-85153485564
OpenAlex:	W4367056546

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