A Knockout of the IFITM3 Gene Increases the Sensitivity of WI-38 VA13 Cells to the Influenza A Virus

A Knockout of the IFITM3 Gene Increases the Sensitivity of WI-38 VA13 Cells to the Influenza A Virus Full article

Journal

International Journal of Molecular Sciences
, E-ISSN: 1422-0067

Output data

Year: 2024, Volume: 25, Number: 1, Article number : 625, Pages count : DOI: 10.3390/ijms25010625

Tags

IFITM; influenza A virus; human cells; WI-38; genome editing; CRISPR/Cas9; RNA-seq

Authors

Eshchenko Natalya ¹ , Sergeeva Mariia ^1,2 , Zhuravlev Evgenii ¹ , Kudria Kira ² , Goncharova Elena ¹ , Komissarov Andrey ^1,2 , Stepanov Grigory ¹

Affiliations

1	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russia
2	Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian Federation, St. Petersburg 197376, Russia

One of the ways to regulate the sensitivity of human cells to the influenza virus is to knock out genes of the innate immune response. Promising targets for the knockout are genes of the interferon-inducible transmembrane protein (IFITM) family, in particular the IFITM3 gene, whose product limits the entry of a virus into the cell by blocking the fusion of the viral and endosomal membranes. In this study, by means of genome-editing system CRISPR/Cas9, monoclonal cell lines with an IFITM3 knockout were obtained based on WI-38 VA13 cells (human origin). It was found that such cell lines are more sensitive to infection by influenza A viruses of various subtypes. Nevertheless, this feature is not accompanied by an increased titer of newly formed viral particles in a culture medium

Eshchenko N. , Sergeeva M. , Zhuravlev E. , Kudria K. , Goncharova E. , Komissarov A. , Stepanov G.
A Knockout of the IFITM3 Gene Increases the Sensitivity of WI-38 VA13 Cells to the Influenza A Virus
International Journal of Molecular Sciences. 2024. V.25. N1. 625 . DOI: 10.3390/ijms25010625 WOS Scopus РИНЦ OpenAlex

Published print:

Jan 3, 2024

Web of science:	WOS:001140458000001
Scopus:	2-s2.0-85182160325
Elibrary:	65717723
OpenAlex:	W4390541903

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