Factors Affecting the Stability of the Trimer of 2'-Deoxyuridine 5'-Triphosphate Nucleotide Hydrolase from Escherichia coli Научная публикация
| Журнал |
Molecular Biology
ISSN: 0026-8933 |
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| Вых. Данные | Год: 2023, Том: 57, Номер: 2, Страницы: 312-319 Страниц : 8 DOI: 10.1134/s002689332302022x | ||||||
| Ключевые слова | dUTPase, protein–protein interactions, oligomerization, differential scanning fluorimetry | ||||||
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Реферат:
—2′-Deoxyuridine 5′-triphosphate nucleotide hhydrolase (Dut) hydrolyzes dUTP to dUMP and
pyrophosphate to prevent erroneous incorporation of dUMP from the dUTP metabolic pool into DNA. Dut
is considered as a promising pharmacological target for antimetabolite therapy. Enzymatically active Dut is a
trimer that binds the substrate at the interface between the subunits. High-speed nanoscale differential scanning fluorimetry (nanoDSF) was used to study how various physicochemical factors affect the stability of the
Escherichia coli Dut trimer. Unlike with monomeric proteins, thermal unfolding of Dut occurred in two steps,
the first one corresponding to dissociation of the trimer into monomeric subunits. Hydrophobic interactions
and hydrogen bonds at the interfaces between the subunits were found to contribute most to trimer stabilization. The binding of nucleotide ligands partly stabilized the Dut trimer. In general, nanoDSF is a convenient
assay for screening low-molecular-weight compounds for their ability to destabilize the active Dut trimer
Библиографическая ссылка:
Yudkina A.V.
, Kovalenko E.A.
, Endutkin A.V.
, Panferova E.P.
, Kirilenko A.A.
, Kokhanenko A.A.
, Zharkov D.O.
Factors Affecting the Stability of the Trimer of 2'-Deoxyuridine 5'-Triphosphate Nucleotide Hydrolase from Escherichia coli
Molecular Biology. 2023. V.57. N2. P.312-319. DOI: 10.1134/s002689332302022x WOS Scopus OpenAlex
Factors Affecting the Stability of the Trimer of 2'-Deoxyuridine 5'-Triphosphate Nucleotide Hydrolase from Escherichia coli
Molecular Biology. 2023. V.57. N2. P.312-319. DOI: 10.1134/s002689332302022x WOS Scopus OpenAlex
Оригинальная:
Юдкина А.В.
, Коваленко Е.А.
, Ендуткин А.В.
, Панферова Е.П.
, Кириленко А.А.
, Коханенко А.А.
, Жарков Д.О.
Факторы, влияющие на стабильность тримерной формы 2′-дезоксиуридин-5′-трифосфатнуклеотидгидролазы Escherichia coli
Молекулярная биология. 2023. Т.57. №2. С.330-339. DOI: 10.31857/S0026898423020246 РИНЦ OpenAlex
Факторы, влияющие на стабильность тримерной формы 2′-дезоксиуридин-5′-трифосфатнуклеотидгидролазы Escherichia coli
Молекулярная биология. 2023. Т.57. №2. С.330-339. DOI: 10.31857/S0026898423020246 РИНЦ OpenAlex
Даты:
| Опубликована в печати: | 16 авг. 2022 г. |
Идентификаторы БД:
| Web of science: | WOS:000984409600016 |
| Scopus: | 2-s2.0-85156136183 |
| OpenAlex: | W4367056758 |