Extracellular vesicles of the liver fluke Opisthorchis felineus stimulate the angiogenesis of human umbilical vein endothelial cells

Extracellular vesicles of the liver fluke Opisthorchis felineus stimulate the angiogenesis of human umbilical vein endothelial cells Full article

Journal

Current Research in Parasitology & Vector-Borne Diseases
ISSN: 2667-114X

Output data

Year: 2023, Volume: 4, Article number : 100153, Pages count : DOI: 10.1016/j.crpvbd.2023.100153

Tags

Extracellular vesiclesHelminthOpisthorchis felineusTranscriptomeHuman vascular endothelial cells

Authors

Ponomarev Dmitry V. ¹ , Lishai Ekaterina A. ¹ , Kovner Anna V. ¹ , Kharkova Maria V. ² , Zaparina Oxana ¹ , Kapuschak Yaroslav K. ¹ , Mordvinov Viatcheslav A. ¹ , Pakharukova Maria Y. ^3,1,4

Affiliations

1	Institute of Cytology and Genetics (ICG), Siberian Branch of the Russian Academy of Sciences (SB RAS), 10 Akad. Lavrentiev Ave., Novosibirsk, 630090, Russia
2	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 8 Akad. Lavrentiev Ave., Novosibirsk, 630090, Russia
3	Department of Natural Sciences, Novosibirsk State University, 2 Pirogova Str., Novosibirsk, 630090, Russia
4	Institute of Molecular Biology and Biophysics of Federal Research Center of Fundamental and Translational Medicine, 2 Timakova Str., Novosibirsk, 630117, Russia

The liver fluke Opisthorchis felineus is a clinically important food-borne parasite of humans. Infection with O. felineus in mammals is associated with liver morbidities such as periductal fibrosis, bile duct neoplasia, and chronic inflammation. Previously we have shown that excretory-secretory products (ESP) can stimulate the healing of skin wounds in mice, which may be due to stimulated angiogenesis and extracellular matrix remodeling. However, there are no studies analyzing the angiogenic character of O. felineus, and its effects on angiogenesis, vascularity, and vascular endothelium. The aim of this study was to evaluate the capacity of ESP and extracellular vesicles (EVs) of O. felineus to stimulate angiogenesis and the formation of pseudo-capillaries in vitro. We also aimed at the assessment of the angiogenesis during the infection in vivo, and estimation of the endothelial cell type abundances from heterogeneous bulk liver transcriptome between uninfected and infected animals with single-cell information. The study revealed significant alterations in vascularity in the hamster liver and significant involvement of portal endothelial cells at the transcriptome level. We also demonstrated that the ESP and EVs of O. felineus have the capacity to stimulate the formation of pseudo-capillaries in vitro. Both ESP and EVs appeared to have similar effects on all four parameters, increasing node formation and total master segments length, and significantly decreasing total isolated branches length and number of isolated segments of pseudo-capillaries. The liver flukes manipulate the hostʼs angiogenic response, a fact that has been related to the pathogenesis caused by these parasites. Understanding these pathogenic mechanisms may uncover new therapeutic targets to relieve or prevent the most severe complications of opisthorchiasis.

Ponomarev D.V. , Lishai E.A. , Kovner A.V. , Kharkova M.V. , Zaparina O. , Kapuschak Y.K. , Mordvinov V.A. , Pakharukova M.Y.
Extracellular vesicles of the liver fluke Opisthorchis felineus stimulate the angiogenesis of human umbilical vein endothelial cells
Current Research in Parasitology & Vector-Borne Diseases. 2023. V.4. 100153 . DOI: 10.1016/j.crpvbd.2023.100153 WOS Scopus OpenAlex

Published print:

Nov 7, 2023

Web of science:	WOS:001119333900001
Scopus:	2-s2.0-85178900299
OpenAlex:	W4388458527

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