Sensing of DNA modifications by pAgo proteins in vitro Научная публикация
Журнал |
Biochimie
ISSN: 0300-9084 |
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Вых. Данные | Год: 2024, Том: 220, Страницы: 39-47 Страниц : 9 DOI: 10.1016/j.biochi.2023.12.006 | ||||
Ключевые слова | Programmable nuclease Prokaryotic argonaute DNA modifications Guide DNA DNA repair | ||||
Авторы |
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Организации |
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Реферат:
Many prokaryotic Argonaute (pAgo) proteins act as programmable nucleases that use small guide DNAs
for recognition and cleavage of complementary target DNA. Recent studies suggested that pAgos
participate in cell defense against invader DNA and may also be involved in other genetic processes,
including DNA replication and repair. The ability of pAgos to recognize specific targets potentially make
them an invaluable tool for DNA manipulations. Here, we demonstrate that DNA-guided DNA-targeting
pAgo nucleases from three bacterial species, DloAgo from Dorea longicatena, CbAgo from Clostridium
butyricum and KmAgo from Kurthia massiliensis, can sense site-specific modifications in the target DNA,
including 8-oxoguanine, thymine glycol, ethenoadenine and pyrimidine dimers. The effects of DNA
modifications on the activity of pAgos strongly depend on their positions relative to the site of cleavage
and are comparable to or exceed the effects of guide-target mismatches at corresponding positions. For
all tested pAgos, the strongest effects are observed when DNA lesions are located at the cleavage position.
The results demonstrate that DNA cleavage by pAgos is strongly affected by DNA modifications, thus
making possible their use as sensors of DNA damage.
Библиографическая ссылка:
Beskrovnaia M.
, Agapov A.
, Makasheva K.
, Zharkov D.O.
, Esyunina D.
, Kulbachinskiy A.
Sensing of DNA modifications by pAgo proteins in vitro
Biochimie. 2024. V.220. P.39-47. DOI: 10.1016/j.biochi.2023.12.006 WOS Scopus OpenAlex
Sensing of DNA modifications by pAgo proteins in vitro
Biochimie. 2024. V.220. P.39-47. DOI: 10.1016/j.biochi.2023.12.006 WOS Scopus OpenAlex
Даты:
Опубликована в печати: | 19 дек. 2023 г. |
Идентификаторы БД:
Web of science: | WOS:001147179700001 |
Scopus: | 2-s2.0-85181256159 |
OpenAlex: | W4389975592 |
Цитирование в БД:
Пока нет цитирований