The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide Nanoparticles Full article
| Journal |
Magnetochemistry
ISSN: 2312-7481 |
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| Output data | Year: 2021, Volume: 7, Number: 9, Article number : 128, Pages count : DOI: 10.3390/magnetochemistry7090128 | ||
| Tags | iron oxide nanoparticles; functionalization; coating; nanomedicine; DNA/gene delivery systems; toxicity; biostability | ||
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Abstract:
Magnetic Fe3O4 nanoparticles (MNPs) have great potential in the nucleic acid delivery approach for therapeutic applications. Herein, the formation of a stable complex of iron oxide nanoparticles with oligonucleotides was investigated. Several factors, such as pH, buffer components, and oligonucleotides sequences, were chosen for binding efficiency studies and oligonucleotide binding constant calculation. Standard characterization techniques, such as dynamic light scattering, zeta potential, and transmission electron microscopy, provide MNPs coating and stability. The toxicity experiments were performed using lung adenocarcinoma A549 cell line and high reactive oxygen species formation with methylene blue assay. Fe3O4 MNPs complexes with oligonucleotides show high stability and excellent biocompatibility.
Cite:
Bobrikova E.
, Chubarov A.
, Dmitrienko E.
The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide Nanoparticles
Magnetochemistry. 2021. V.7. N9. 128 . DOI: 10.3390/magnetochemistry7090128 WOS Scopus РИНЦ OpenAlex
The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide Nanoparticles
Magnetochemistry. 2021. V.7. N9. 128 . DOI: 10.3390/magnetochemistry7090128 WOS Scopus РИНЦ OpenAlex
Dates:
| Published print: | Sep 14, 2021 |
Identifiers:
| Web of science: | WOS:000699580200001 |
| Scopus: | 2-s2.0-85115292472 |
| Elibrary: | 47011794 |
| OpenAlex: | W3201010329 |