Abstract: A protein corona is present on any nanoparticle (NP) entering biological fluids; however, the existence of a natural protein corona on natural NPs has not been experimentally confirmed. We used our previously developed photomodification method to fix the natural corona on “biological nanoparticles” (bio-NPs) in fetal bovine serum and newborn bovine serum; native sera served as a control. To isolate photomodified bio-NPs, we used ultracentrifugation (UC), sucrose gradient (12%, 30%, and 50%), and sucrose cushion (30%) methods. Isolated bio-NPs were visualized using transmission electron microscopy. The results showed that a protein corona is present on extracellular vesicles and lipoproteins isolated by UC. The isolation of bio-NPs through sucrose gradient or cushion did not preserve the protein corona. At the same time, we observed signs of a negative effect of the sucrose gradient on bio-NPs of intact and photomodified serum; when isolating these particles on a sucrose cushion, no negative effects were observed. We believe that the data we present will be useful to researchers using sucrose solutions to isolate bio-NPs and working on the properties of the protein corona. In this work, we have obtained direct images of a “natural” protein corona on natural bio-NPs of blood serum for the first time

Cite: Poletaeva Y.E. , Tupitsyna A.V. , Grigoryeva A.E. , Dovydenko I.S. , Ryabchikova E.I.
Attempts to preserve and visualize protein corona on the surface of biological nanoparticles in blood serum using photomodification
Beilstein Journal of Nanotechnology. 2024. V.15. P.1654–1666. DOI: 10.3762/bjnano.15.130 OpenAlex

Dates:

Published print:

Dec 30, 2024

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OpenAlex:

W4405914224

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