Abstract: Small interfering RNA (siRNA) is the most important tool for the manipulation of mRNA expression and needs protection from intracellular nucleases when delivered into the cell. In this work, we examined the effects of siRNA modification with the phosphoryl guanidine (PG) group, which, as shown earlier, makes oligodeoxynucleotides resistant to snake venom phosphodiesterase. We obtained a set of siRNAs containing combined modifications PG/2′-O-methyl (2′-OMe) or PG/2′-fluoro (2′-F); biophysical and biochemical properties were characterized for each duplex. We used the UV-melting approach to estimate the thermostability of the duplexes and RNAse A degradation assays to determine their stability. The ability to induce silencing was tested in cultured cells stably expressing green fluorescent protein. The introduction of the PG group as a rule decreased the thermodynamic stability of siRNA. At the same time, the siRNAs carrying PG groups showed increased resistance to RNase A. A gene silencing experiment indicated that the PG-modified siRNA retained its activity if the modifications were introduced into the passenger strand. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Cite: Pavlova A.S. , Yakovleva K.I. , Epanchitseva A.V. , Kupryushkin M.S. , Pyshnaya I.A. , Pyshnyi D.V. , Ryabchikova E.I. , Dovydenko I.S.
An influence of modification with phosphoryl guanidine combined with a 2′-o-methyl or 2′-fluoro group on the small-interfering-rna effect
International Journal of Molecular Sciences. 2021. V.22. N18. 9784 . DOI: 10.3390/ijms22189784 WOS Scopus РИНЦ OpenAlex

Dates:

Published print:

Sep 10, 2021

Identifiers:

Web of science:	WOS:000699758900001
Scopus:	2-s2.0-85114608355
Elibrary:	47078879
OpenAlex:	W3200824188

Citing:

DB	Citing
Scopus	7
OpenAlex	9
Web of science	6

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