Triazinylamidophosphate Oligonucleotides: Synthesis and Study of Their Interaction with Cells and DNA-Binding Proteins Научная публикация
| Журнал |
Russian Journal of Bioorganic Chemistry
ISSN: 1068-1620 |
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| Вых. Данные | Год: 2021, Том: 47, Номер: 3, Страницы: 719-733 Страниц : 15 DOI: 10.1134/S1068162021030110 | ||||
| Ключевые слова | automated amidophosphite synthesis; Ku-antigen; PARP1; Staudinger’s reaction; triazinylamidophosphate oligonucleotides | ||||
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Реферат:
Abstract: In this work, representatives of the class of triazinylamidophosphate oligonucleotide derivatives were obtained for the first time. A scheme for the introduction of a modified unit on a solid-phase support during the oxidation step with highly reactive 2-azido-4,6-dichloro[1,3,5]triazine according to the Staudinger reaction was proposed and implemented, followed by the introduction of various aliphatic residues upon treatment with the corresponding amines. A number of model oligonucleotides containing triazinylamidophosphate modification were synthesized. Chemical stability, cellular uptake, and cytotoxicity of the obtained derivatives were studied. The effect of the type of aliphatic substituent in the composition of the oligonucleotides obtained on their resistance to enzymatic hydrolysis in extracts of cultured human cells with different contents of key DNA repair regulatory proteins, Ku-antigen and poly(ADP-ribose) polymerase 1 (PARP1), which are potentially the most effective acceptors of modified oligonucleotides, was studied. © 2021, Pleiades Publishing, Ltd.
Библиографическая ссылка:
Kupryushkin M.S.
, Zharkov T.D.
, Ilina E.S.
, Markov O.V.
, Kochetkova A.S.
, Akhmetova M.M.
, Lomzov A.A.
, Pyshnyi D.V.
, Lavrik O.I.
, Khodyreva S.N.
Triazinylamidophosphate Oligonucleotides: Synthesis and Study of Their Interaction with Cells and DNA-Binding Proteins
Russian Journal of Bioorganic Chemistry. 2021. V.47. N3. P.719-733. DOI: 10.1134/S1068162021030110 WOS Scopus РИНЦ OpenAlex
Triazinylamidophosphate Oligonucleotides: Synthesis and Study of Their Interaction with Cells and DNA-Binding Proteins
Russian Journal of Bioorganic Chemistry. 2021. V.47. N3. P.719-733. DOI: 10.1134/S1068162021030110 WOS Scopus РИНЦ OpenAlex
Оригинальная:
Купрюшкин М.С.
, Жарков Т.Д.
, Ильина Е.С.
, Марков О.В.
, Кочеткова А.С.
, Ахметова М.М.
, Ломзов А.А.
, Пышный Д.В.
, Лаврик О.И.
, Ходырева С.Н.
Триазиниламидофосфатные олигонуклеотиды: получение и исследование их взаимодействия с клетками и ДНК-связывающими белками
Биоорганическая химия. 2021. Т.47. №3. С.348-364. DOI: 10.31857/S0132342321030118 РИНЦ OpenAlex
Триазиниламидофосфатные олигонуклеотиды: получение и исследование их взаимодействия с клетками и ДНК-связывающими белками
Биоорганическая химия. 2021. Т.47. №3. С.348-364. DOI: 10.31857/S0132342321030118 РИНЦ OpenAlex
Даты:
| Принята к публикации: | 28 окт. 2020 г. |
Идентификаторы БД:
| Web of science: | WOS:000660549500010 |
| Scopus: | 2-s2.0-85107601697 |
| РИНЦ: | 46807447 |
| OpenAlex: | W3166077892 |