Conjugates of RNase P-Guiding Oligonucleotides with Oligo(N-Methylpyrrole) as Prospective Antibacterial Agents

Conjugates of RNase P-Guiding Oligonucleotides with Oligo(N-Methylpyrrole) as Prospective Antibacterial Agents Full article

Journal

Russian Journal of Bioorganic Chemistry
ISSN: 1068-1620

Output data

Year: 2021, Volume: 47, Number: 2, Pages: 469-477 Pages count : 9 DOI: 10.1134/S1068162021020084

Tags

bacterial RNAse P; EGS-oligonucleotides; oligo(2'-O-methylribonucleotides); oligo(N-methylpyrrole)

Authors

Danilin N.A. ^1,2 , Matveev A.L. ² , Tikunova N.V. ² , Venyaminova A.G. ² , Novopashina D.S. ^1,2

Affiliations

1	Novosibirsk State University, Novosibirsk, 630090, Russian Federation
2	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russian Federation

Abstract: The conjugates of RNase P guiding oligo(2'-O-methylribo)- and oligodeoxyribonucleotides (EGS oligonucleotides) with oligo(N-methylpyrrole) have been synthesized for the first time. The ability of RNase P to hydrolyze RNA in the presence of EGS oligonucleotides and their conjugates with oligo(N-methylpyrrole) has been demonstrated in model systems using fluorescently labeled chemically synthesized oligoribonucleotides corresponding to the fragments of mRNA of the ftsZ and gyrA genes of Acinetobacter baumannii. It has been shown that hydrolysis of RNA by RNase P occurs more efficiently in the presence of the conjugates than in the case of unmodified oligodeoxyribonucleotides. The introduction of oligo(N-methylpyrrole) in EGS oligo(2'-O-methylribonucleotides) insignificantly changes the effectiveness of hydrolysis. It has been demonstrated that the cell-penetrating ability of oligonucleotides is enhanced because of the presence of oligo(N-methylpyrrole) at the 5'-end. © 2021, Pleiades Publishing, Ltd.

Danilin N.A. , Matveev A.L. , Tikunova N.V. , Venyaminova A.G. , Novopashina D.S.
Conjugates of RNase P-Guiding Oligonucleotides with Oligo(N-Methylpyrrole) as Prospective Antibacterial Agents
Russian Journal of Bioorganic Chemistry. 2021. V.47. N2. P.469-477. DOI: 10.1134/S1068162021020084 WOS Scopus OpenAlex

Данилин Н.А. , Матвеев А.Л. , Тикунова Н.В. , Веньяминова А.Г. , Новопашина Д.С.
Направляющие РНКазу Р конъюгаты олигонуклеотидов с олиго(N-метилпирролом) как перспективные антибактериальные препараты
Биоорганическая химия. 2021. Т.47. №2. С.259-269. DOI: 10.31857/S0132342321020081 РИНЦ OpenAlex

Web of science:	WOS:000644646500014
Scopus:	2-s2.0-85104884955
OpenAlex:	W3161634691

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