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Photoactivatable CRISPR/Cas9 System Full article

Journal Russian Journal of Bioorganic Chemistry
ISSN: 1068-1620
Output data Year: 2021, Volume: 47, Number: 2, Pages: 496-504 Pages count : 9 DOI: 10.1134/S1068162021020023
Tags guide RNAs for CRISPR/Cas9 system; photoactivatable gene editing in vitro; photoblocking oligodeoxyribonucleotide; photocleavable linker
Authors Akhmetova E.A. 1,2,3 , Golyshev V.M. 1,3 , Vokhtantcev I.P. 1,3 , Meschaninova M.I. 1 , Venyaminova A.G. 1 , Novopashina D.S. 1,2,3
Affiliations
1 Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russian Federation
2 Sirius University of Science and Technology, Sochi, 354383, Russian Federation
3 Novosibirsk State University, Novosibirsk, 630090, Russian Federation

Abstract: Abstract: A photoactivatable CRISPR/Cas9 system consisting of the Cas9 protein, synthetic 102-nt sgRNA or a pair of guide crRNA/tracrRNA, and blocking photocleavable oligodeoxyribonucleotides has been designed. Photocleavable oligodeoxyribonucleotides contain two or three photodegradable linkers in their structure. The thermal stability of complexes of these modified oligonucleotides with guide crRNA decreases with increasing the number of linkers. The kinetics of the photomodified oligodeoxyribonucleotides cleavage has been investigated. It has been shown that UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can result in photoactivatable gene editing in vitro. © 2021, Pleiades Publishing, Ltd.
Cite: Akhmetova E.A. , Golyshev V.M. , Vokhtantcev I.P. , Meschaninova M.I. , Venyaminova A.G. , Novopashina D.S.
Photoactivatable CRISPR/Cas9 System
Russian Journal of Bioorganic Chemistry. 2021. V.47. N2. P.496-504. DOI: 10.1134/S1068162021020023 WOS Scopus OpenAlex
Original: Ахметова Е.А. , Голышев В.М. , Вохтанцев И.П. , Мещанинова М.И. , Веньяминова А.Г. , Новопашина Д.С.
Фотоактивируемая система CRISPR/CAS9
Биоорганическая химия. 2021. Т.47. №2. С.276-286. DOI: 10.31857/S0132342321020020 РИНЦ OpenAlex
Identifiers:
Web of science: WOS:000644646500017
Scopus: 2-s2.0-85104853571
OpenAlex: W3176111215
Citing:
DB Citing
Scopus 4
Web of science 5
OpenAlex 5
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