New Two-Component Pyrene Probes Based on Oligo(2'-O-Methylribonucleotides) for microRNA Detection

New Two-Component Pyrene Probes Based on Oligo(2'-O-Methylribonucleotides) for microRNA Detection Научная публикация

Журнал

Russian Journal of Bioorganic Chemistry
ISSN: 1068-1620

Вых. Данные

Год: 2021, Том: 47, Номер: 2, Страницы: 432-440 Страниц : 9 DOI: 10.1134/S1068162021020230

Ключевые слова

PyA-modified adenine cluster; 3WJ structure; 8-(1-ethynylpyrene)-deoxyriboadenosine; fluorescent properties; oligo(2'-O-methylribonucleotides); RNA detection

Авторы

Semikolenova O.A. ^1,2 , Golyshev V.M. ¹ , Kim B.H. ³ , Venyaminova A.G. ¹ , Novopashina D.S. ^1,2

Организации

1	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russian Federation
2	Novosibirsk State University, Novosibirsk, 630090, Russian Federation
3	Pohang University of Science and Technology (POSTECH), Hyogok-dong, Nam-gu, Pohang-si, Gyeongsangbuk-do, South Korea

Abstract: New two-component pyrene probes based on oligo(2'-O-methylribonucleotides) for microRNA detection have been designed. They contain the PyA-modified adenine cluster (pentaadenosine fragment that contains 8-(1-ethynylpyrene)-deoxyriboadenosine in the center) and can form a three-way junction (3WJ) structure with a target RNA. We have chosen microRNA let-7a-3p as the RNA target because of the correlation of its concentration in cells with the appearance and progression of cancer. We have compared the thermal stability and fluorescence properties of the two-component probes based on oligo(2'-O-methylribonucleotides) that contain either deoxyriboadenosine (dAdAPyAdAdA) or the (2'-O-methylribo)adenosine (AmAm PyAAmAm) cluster with those of (dAdAPyAdAdA)-containing oligodeoxyribonucleotide. The changes in the fluorescence spectra of two-component probes after hybridization with the RNA target have been demonstrated. These probes can be used for designing a new microRNA detection system. © 2021, Pleiades Publishing, Ltd.

Semikolenova O.A. , Golyshev V.M. , Kim B.H. , Venyaminova A.G. , Novopashina D.S.
New Two-Component Pyrene Probes Based on Oligo(2'-O-Methylribonucleotides) for microRNA Detection
Russian Journal of Bioorganic Chemistry. 2021. V.47. N2. P.432-440. DOI: 10.1134/S1068162021020230 WOS Scopus OpenAlex

Семиколенова О.А. , Голышев В.М. , Ким Б-Х. , Веньяминова А.Г. , Новопашина Д.С.
Новые двухкомпонентные пиренильные зонды на основе олиго(2'-О-метилрибонуклеотидов) для детекции микроРНК
Биоорганическая химия. 2021. Т.47. №2. С.218-227. DOI: 10.31857/S0132342321020238 РИНЦ OpenAlex

Web of science:	WOS:000644646500010
Scopus:	2-s2.0-85104822304
OpenAlex:	W3166893700

БД	Цитирований
Web of science	1
OpenAlex	1