Abstract: This work aimed at developing and validating a rapid, sensitive, and robust method of liquid chromatography with high‐resolution mass spectrometry (LC–HRMS) in parallel reaction monitoring (PRM) mode for apixaban quantification in dried plasma spots (DPSs) with a simple extraction procedure. A 25 μL sample of human plasma was placed onto Whatman 903 Protein Saver Cards and allowed to dry; 3.2 mm diameter disks were cut out from DPSs using a puncher, and 100 μL of a working internal standard solution was added to each sample. After this, they were vortexed on a shaker for 15 min at 800 rpm and 40 °C and quick centrifugation (10,000× g, 10 s), and then the extracts were transferred into a 300 μL vial for LC–HRMS. Data were acquired in PRM mode via detection of all target product ions with 10 ppm tolerance. Total analysis time was 5 min. The LC–HRMS method was validated for the 10–400 ng/mL range with R2 > 0.99. Within this range, intra‐ and interday variability of precision and accuracy was <10%, and recovery was 69.7–85.1%. Apixaban was stable after brief storage at room tem-perature, and at 4 °C for up to a month. The method development and validation results proved that this LC–HRMS assay of apixaban in DPSs is selective and robust. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Cite: Chernonosov A. , Aksenova L. , Koval V.
The development of a liquid chromatography high‐resolution mass spectrometric method for apixaban quantification in dried plasma spots in parallel reaction monitoring mode
Processes. 2021. V.9. N3. 450 :1-12. DOI: 10.3390/pr9030450 WOS Scopus РИНЦ OpenAlex

Dates:

Published print:

Mar 2, 2021

Identifiers:

Web of science:	WOS:000634194300001
Scopus:	2-s2.0-85102530418
Elibrary:	46767253
OpenAlex:	W3134528477

Citing:

DB	Citing
Scopus	2
Elibrary	1
Web of science	1
OpenAlex	2

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