The Level of LINE-1 mRNA Is Increased in Extracellular Circulating Plasma RNA in Patients with Colorectal Cancer

The Level of LINE-1 mRNA Is Increased in Extracellular Circulating Plasma RNA in Patients with Colorectal Cancer Full article

Journal

Bulletin of Experimental Biology and Medicine
ISSN: 0007-4888

Output data

Year: 2022, Volume: 173, Number: 2, Pages: 261-264 Pages count : 4 DOI: 10.1007/s10517-022-05530-2

Tags

colorectal cancer; freely circulating RNA; LINE-1; long dispersed nucleotide repeats (LINE); transcription

Authors

Filipenko M.L. ¹ , Boyarskikh U.A. ¹ , Leskov L.S. ⁴ , Subbotina K.V. ¹ , Khrapov E.A. ¹ , Sokolov A.V. ³ , Stilidi I.S. ² , Kushlinskii N.E. ²

Affiliations

1	Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russian Federation
2	N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation
3	City Clinical Hospital No. 1, Novosibirsk, Russian Federation
4	M. F. Vladimirsky Moscow Regional Research Clinical Institute, Moscow, Russian Federation

We performed a comparative quantitative analysis of LINE-1 mRNA levels in extracellular total plasma RNA in patients with colon cancer and practically healthy donors. Quantitative multiplex PCR with reverse transcription was used to assess the level of LINE-1 and 18S rRNA mRNA in extracellular total plasma RNA. The median of LINE-1 mRNA values in colon cancer patients (4.95) was significantly higher than in healthy donors (2.3) (p=0.037). It was shown for the first time that the level of LINE-1 mRNA in total RNA from blood plasma can be determined in the format of a liquid biopsy and serve as a new potential non-invasive marker of colon cancer. © 2022, Springer Science+Business Media, LLC, part of Springer Nature.

Filipenko M.L. , Boyarskikh U.A. , Leskov L.S. , Subbotina K.V. , Khrapov E.A. , Sokolov A.V. , Stilidi I.S. , Kushlinskii N.E.
The Level of LINE-1 mRNA Is Increased in Extracellular Circulating Plasma RNA in Patients with Colorectal Cancer
Bulletin of Experimental Biology and Medicine. 2022. V.173. N2. P.261-264. DOI: 10.1007/s10517-022-05530-2 WOS Scopus OpenAlex

Филипенко М.Л. , Боярских У.А. , Лесков Л.С. , Субботина К.В. , Храпов Е.А. , Соколов А.В. , Стилиди И.С. , Кушлинский Н.Е.
Уровень мРНК LINE-1 повышен в свободно циркулирующей РНК плазмы крови у больных колоректальным раком
БЮЛЛЕТЕНЬ ЭКСПЕРИМЕНТАЛЬНОЙ БИОЛОГИИ И МЕДИЦИНЫ. 2022. Т.173. №2. С.242-245. DOI: 10.47056/0365-9615-2022-173-2-242-245 РИНЦ OpenAlex

Web of science:	WOS:000814938400019
Scopus:	2-s2.0-85132575299
OpenAlex:	W4283322837

DB	Citing
Scopus	2
Web of science	1
OpenAlex	1