Реферат: In this study, we reported a rapid, sensitive, robust, and validated method for atenolol quantification in dried plasma spots (DPS) by liquid chromatography with high-resolution mass spectrometry (LC-HRMS) using parallel reaction monitoring mode (PRM). Aliquots of 25 µL human plasma were placed onto Whatman 903 Cards and air-dried. Disks (3.2 mm internal diameter) were punched, and a 100 µL working internal standard solution was added to each sample and then incubated on a shaker for 15 min at 40 °C, followed by rapid centrifugation (10,000× g, 10 s). The supernatant was transferred into 300 µL vials for subsequent LC–HRMS analysis. After chromatographic separation, atenolol and the internal standard were quantified in positive-ion parallel reaction monitoring mode by detection of all target product ions at 10 ppm tolerances. The total time of the analysis was 5 min. The calibration curve was linear in the range of 5–1000 ng/mL with interday and intraday precision levels and biases of <14.4%, and recovery was 62.9–81.0%. The atenolol in DPS was stable for ≥30 days at 25 and 4 °C. This fully validated method is selective and suitable for atenolol quantitation in DPS using LC–HRMS. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Библиографическая ссылка: Aksenova L.V. , Koval V.V. , Chernonosov A.A.
Parallel Reaction Monitoring Mode for Atenolol Quantification in Dried Plasma Spots by Liquid Chromatography Coupled with High-Resolution Mass Spectrometry
Processes. 2022. V.10. N7. 1240 . DOI: 10.3390/pr10071240 WOS Scopus OpenAlex

Идентификаторы БД:

Web of science:	WOS:000831990700001
Scopus:	2-s2.0-85133012501
OpenAlex:	W4283268045

Цитирование в БД:

БД	Цитирований
Scopus	1
Web of science	1
OpenAlex	1

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