Stability of ZIF-8 Nanoparticles in Most Common Cell Culture Media

Stability of ZIF-8 Nanoparticles in Most Common Cell Culture Media Full article

Journal

Molecules
ISSN: 1420-3049

Output data

Year: 2022, Volume: 27, Number: 10, Article number : 3240, Pages count : DOI: 10.3390/molecules27103240

Tags

electron paramagnetic resonance (EPR); metal–organic frameworks (MOFs); ZIF-8

Authors

Spitsyna Anna S. ^1,2 , Poryvaev Artem S. ¹ , Sannikova Natalya E. ¹ , Yazikova Anastasiya A. ¹ , Kirilyuk Igor A. ² , Dobrynin Sergey A. ² , Chinak Olga A. ³ , Fedin Matvey V. ¹ , Krumkacheva Olesya A. ¹

Affiliations

1	International Tomography Center SB RAS, Novosibirsk 630090, Russia
2	N.Vorozhtsov Institute of Organic Chemistry SB RAS, Novosibirsk 630090, Russia
3	Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk 630090, Russia

Zeolite imidazolate framework-8 (ZIF-8) is a promising platform for drug delivery, and information regarding the stability of ZIF-8 nanoparticles in cell culture media is essential for proper interpretation of in vitro experimental results. In this work, we report a quantitative investigation of the ZIF-8 nanoparticle’s stability in most common cell culture media. To this purpose, ZIF-8 nanoparticles containing sterically shielded nitroxide probes with high resistance to reduction were synthesized and studied using electron paramagnetic resonance (EPR). The degradation of ZIF-8 in cell media was monitored by tracking the cargo leakage. It was shown that nanoparticles degrade at least partially in all studied media, although the degree of cargo leakage varies widely. We found a strong correlation between the amount of escaped cargo and total concentration of amino acids in the environment. We also established the role of individual amino acids in ZIF-8 degradation. Finally, 2-methylimidazole preliminary dissolved in cell culture media partially inhibits the degradation of ZIF-8 nanoparticles. The guidelines for choosing the proper cell culture medium for the in vitro study of ZIF-8 nanoparticles have been formulated. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Spitsyna A.S. , Poryvaev A.S. , Sannikova N.E. , Yazikova A.A. , Kirilyuk I.A. , Dobrynin S.A. , Chinak O.A. , Fedin M.V. , Krumkacheva O.A.
Stability of ZIF-8 Nanoparticles in Most Common Cell Culture Media
Molecules. 2022. V.27. N10. 3240 . DOI: 10.3390/molecules27103240 WOS Scopus OpenAlex

Web of science:	WOS:000804880100001
Scopus:	2-s2.0-85130577304
OpenAlex:	W4280654647

DB	Citing
Scopus	20
Web of science	27
OpenAlex	30