Validation of Heterophase RNA Analysis with the Use of a Silicon-on-Insulator Biosensor

Validation of Heterophase RNA Analysis with the Use of a Silicon-on-Insulator Biosensor Full article

Journal

Optoelectronics, Instrumentation and Data Processing
ISSN: 8756-6990

Output data

Year: 2021, Volume: 57, Number: 1, Pages: 44-50 Pages count : 7 DOI: 10.3103/S8756699021010039

Tags

electroneutral phosphoryl guanidine oligonucleotides; heterophase analysis; hybridization; Si/SiO2 surfaces; SOI biosensor

Authors

Dmitrienko E.V. ^1,2 , Poryvaeva A.V. ¹ , Naumova O.V. ³ , Fomin B.I. ³ , Kupryushkin M.S. ¹ , Pyshnaya I.A. ¹ , Pyshnyi D.V. ^1,2

Affiliations

1	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russian Federation
2	Novosibirsk State University, Novosibirsk, 630090, Russian Federation
3	Rzhanov Institute of Semiconductor Physics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russian Federation

Abstract: The analysis of nucleic acids remains to be a topical trend in the development of medical diagnostics. Contemporary ultrasensitive diagnostic systems provide the conversion of a specific interaction into a hardwarely-detectable signal in the course of analysis. An example of such diagnostic devices is silicon-on-insulator (SOI) biosensors of field-effect transistors (FET). In this study, glass surfaces are used (as a Si/SiO2 surface for a SOI biosensor) for the optimization and validation of all analysis stages. The efficient immobilization of electroneutral analogs of oligonucleides onto a Si/SiO2surface after the activation of Si-OH groups with 3-glycidoxypropyltrimethoxysilane or carbonyldiimidazole is demonstrated. In the presence of salt at a low concentration or with no salt, the possibility of detecting an RNA target on model glass surfaces in the parallel analysis regime is shown. The regeneration of the Si/SiO2 surface of sensors for repeated analysis and the stability of sensors under long-term storage are also demonstrated. © 2021, Allerton Press, Inc.

Dmitrienko E.V. , Poryvaeva A.V. , Naumova O.V. , Fomin B.I. , Kupryushkin M.S. , Pyshnaya I.A. , Pyshnyi D.V.
Validation of Heterophase RNA Analysis with the Use of a Silicon-on-Insulator Biosensor
Optoelectronics, Instrumentation and Data Processing. 2021. V.57. N1. P.44-50. DOI: 10.3103/S8756699021010039 WOS Scopus РИНЦ OpenAlex

Дмитриенко Е.В. , Порываева А.В. , Наумова О.В. , Фомин Б.И. , Купрюшкин М.С. , Пышная И.А. , Пышный Д.В.
Валидация гетерофазного анализа РНК с помощью КНИ-биосенсора
Автометрия. 2021. Т.57. №№ 1. С.50-56. DOI: 10.15372/AUT20210106 РИНЦ OpenAlex

Web of science:	WOS:000672465500006
Scopus:	2-s2.0-85109932896
Elibrary:	46889925
OpenAlex:	W3180806175

DB	Citing
Scopus	1
Elibrary	1
Web of science	1
OpenAlex	1