Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments

Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments Научная публикация

Журнал

Bulletin of Experimental Biology and Medicine
ISSN: 0007-4888

Вых. Данные

Год: 2022, Том: 173, Номер: 3, Страницы: 384-389 Страниц : 6 DOI: 10.1007/s10517-022-05554-8

Ключевые слова

isopropanol; lithium chloride; precipitation selectivity; reverse transcription; RNA isolation

Авторы

Korolenya V.A. ^1,2 , Gavrilov K.A. ^1,2 , Sevost’ianova K.S. ^1,2 , Shevela A.I. ^1,2 , Filipenko M.L. ^1,2 , Smetanina M.A. ^1,2

Организации

1	Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russian Federation
2	Novosibirsk National Research State University, Novosibirsk, Russian Federation

Contamination of the sample with DNA is a problem when isolating RNA by phenol-chloroform extraction, and DNase treatment introduces an additional error in the analysis of gene expression. Bearing in mind the ability of LiCl to precipitate RNA selectively, we studied the possibility and advantage of using LiCl as a precipitation agent in the protocol for RNA precipitation [3] from frozen segments of large vessels. Combined use of LiCl with isopropanol as precipitating agents (optimal ratio 2.5 M and 40%, respectively) significantly eliminates negative effects of using this salt (inhibition of reverse transcription and low RNA yield). © 2022, Springer Science+Business Media, LLC, part of Springer Nature.

Korolenya V.A. , Gavrilov K.A. , Sevost’ianova K.S. , Shevela A.I. , Filipenko M.L. , Smetanina M.A.
Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments
Bulletin of Experimental Biology and Medicine. 2022. V.173. N3. P.384-389. DOI: 10.1007/s10517-022-05554-8 WOS Scopus OpenAlex

Короленя В.А. , Гаврилов К.А. , Севостьянова К.С. , Шевела А.И. , Филипенко М.Л. , Сметанина М.А.
Оценка преимуществ применения хлорида лития в качестве осаждающего агента при выделении РНК из замороженных сегментов вен
БЮЛЛЕТЕНЬ ЭКСПЕРИМЕНТАЛЬНОЙ БИОЛОГИИ И МЕДИЦИНЫ. 2022. Т.173. №3. С.384-389. DOI: 10.47056/0365-9615-2022-173-3-384-389 РИНЦ OpenAlex

Web of science:	WOS:000827406200006
Scopus:	2-s2.0-85134515587
OpenAlex:	W4285797970

БД	Цитирований
Scopus	1
Web of science	1
OpenAlex	2