Abstract: In this work, we developed and validated a robust and sensitive method of liquid chromatography with high-resolution mass spectrometry in parallel reaction monitoring (PRM) mode for ST-246 (tecovirimat) quantification in human blood plasma. The method was compared with the multiple reaction monitoring (MRM) technique and showed better selectivity and similar sensitivity in a wider concentration range (10–5000 ng/mL). Within this range, intra- and interday variability of precision and accuracy were within acceptable ranges in accordance with the European Medicines Agency guidelines, and recovery was 87.9–100.6%. Samples were stable at 4 °C within 48 h and at −20 °C up to 3 months. The recovery and matrix effects in the proposed HRMS method were about 5% higher than those reported for the MRM method, but the PRM method showed better accuracy with comparable precision. It was found that the ST-246 concentration shown by the PRM method is approximately 24% higher than the output of the MRM one. Nonetheless, the high selectivity with similar sensitivity, as compared with traditional MRM methods, makes the proposed approach attractive for research and clinical use. © 2022 by the authors.

Cite: Chernonosov A.A. , Oleinik G.A. , Koval V.V.
Application of Parallel Reaction Monitoring to the Development and Validation of a Quantitative Assay for ST-246 in Human Plasma
International Journal of Molecular Sciences. 2022. V.23. N14. 8021 . DOI: 10.3390/ijms23148021 WOS Scopus OpenAlex

Dates:

Published print:

Jul 21, 2022

Identifiers:

Web of science:	WOS:000833113700001
Scopus:	2-s2.0-85135120524
OpenAlex:	W4286491118

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Scopus	1
OpenAlex	3
Web of science	4

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