Effect of Fluorescent Labels on DNA Affinity for Gold Nanoparticles Full article
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Nanomaterials
ISSN: 2079-4991 |
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| Output data | Year: 2021, Volume: 11, Number: 5, Article number : 1178, Pages count : DOI: 10.3390/nano11051178 | ||
| Tags | oligonucleotides; fluorescent dye; gold nanoparticles; non-covalent adsorption; mechanisms; affinity; Langmuir constant; fluorophore importance | ||
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Abstract:
Fluorophore (FD) labeling is widely used for detection and quantification of various compounds bound to nanocarriers. The systems, composed of gold nanoparticles (GNPs) and oligonucleotides (ONs) labeled with FDs, have wide applications. Our work was aimed at a systemic study of how FD structure (in composition of ON-FDs) influenced the efficiency of their non-covalent associates' formation with GNPs (ON-FD/GNPs). We examined ONs of different length and nucleotide composition, and corresponding ON-FDs (FDs from a series of xanthene, polymethine dyes; dyes based on polycyclic aromatic hydrocarbons). Methods: fluorometry, dynamic light scattering, high performance liquid chromatography, gel electrophoresis, molecular modeling and methods of thermodynamic and statistical analysis. We observed significant, differing several times, changes in surface density and Langmuir constant values of ON-FDs vs. ONs, evidence for the critical significance of FD nature for binding of ON-FDs with GNPs. Surface density of ON-FD/GNPs; hydrophobicity and total charge of ON or ON-FD; and charge and surface area of FDs were revealed as key factors determining affinity (Langmuir constant) of ON or ON-FDs for GNPs. These factors compose a specific set, which makes possible the highly reliable prediction of efficiency of ONs and ON-FDs binding with GNPs. The principal possibility of creating an algorithm for predictive calculation of efficiency of ONs and GNPs interaction was demonstrated. We proposed a hypothetical model that described the mechanism of contact interaction between negatively charged nano-objects, such as citrate-stabilized GNPs, and ONs or ON-FDs.
Cite:
Epanchintseva A.V.
, Gorbunova E.A.
, Ryabchikova E.I.
, Pyshnaya I.A.
, Pyshnyi D.V.
Effect of Fluorescent Labels on DNA Affinity for Gold Nanoparticles
Nanomaterials. 2021. V.11. N5. 1178 . DOI: 10.3390/nano11051178 WOS Scopus OpenAlex
Effect of Fluorescent Labels on DNA Affinity for Gold Nanoparticles
Nanomaterials. 2021. V.11. N5. 1178 . DOI: 10.3390/nano11051178 WOS Scopus OpenAlex
Dates:
| Published print: | Apr 29, 2021 |
Identifiers:
| Web of science: | WOS:000657005500001 |
| Scopus: | 2-s2.0-85115308806 |
| OpenAlex: | W3158195386 |