Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods Научная публикация
| Журнал |
Diagnostics
ISSN: 2075-4418 |
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| Вых. Данные | Год: 2021, Том: 11, Номер: 5, Номер статьи : 865, Страниц : DOI: 10.3390/diagnostics11050865 | ||||||
| Ключевые слова | extracellular vesicles; lipoproteins; binding to blood cells; cell-free miRNA structure; miRNA storage; isolation kit; miRNA isolation | ||||||
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Реферат:
A vast wealth of recent research has seen attempts of using microRNA (miRNA) found in biological fluids in clinical research and medicine. One of the reasons behind this trend is the apparent their high stability of cell-free miRNA conferred by small size and packaging in supramolecular complexes. However, researchers in both basic and clinical settings often face the problem of selecting adequate methods to extract appropriate quality miRNA preparations for use in specific downstream analysis pipelines. This review outlines the variety of different methods of miRNA isolation from biofluids and examines the key determinants of their efficiency, including, but not limited to, the structural properties of miRNA and factors defining their stability in the extracellular environment.
Библиографическая ссылка:
Bryzgunova O.
, Konoshenko M.
, Zaporozhchenko I.
, Yakovlev A.
, Laktionov P.
Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods
Diagnostics. 2021. V.11. N5. 865 . DOI: 10.3390/diagnostics11050865 WOS Scopus РИНЦ OpenAlex
Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods
Diagnostics. 2021. V.11. N5. 865 . DOI: 10.3390/diagnostics11050865 WOS Scopus РИНЦ OpenAlex
Даты:
| Опубликована в печати: | 11 мая 2021 г. |
Идентификаторы БД:
| Web of science: | WOS:000653792700001 |
| Scopus: | 2-s2.0-85106467082 |
| РИНЦ: | 46087449 |
| OpenAlex: | W3163555187 |