Pectobacterium versatile Bacteriophage Possum: A Complex Polysaccharide-Deacetylating Tail Fiber as a Tool for Host Recognition in Pectobacterial Schitoviridae

Pectobacterium versatile Bacteriophage Possum: A Complex Polysaccharide-Deacetylating Tail Fiber as a Tool for Host Recognition in Pectobacterial Schitoviridae Full article

Journal

International Journal of Molecular Sciences
, E-ISSN: 1422-0067

Output data

Year: 2022, Volume: 23, Number: 19, Pages: 11043 Pages count : 1 DOI: 10.3390/ijms231911043

Tags

bacteriophage; Pectobacterium versatile; phylogeny; polysaccharide; polysaccharide deacetylase; Schitoviridae; soft rot; tail fiber

Authors

Lukianova AA. ^1,2 , Evseev P.V. ¹ , Shneider M.M. ¹ , Dvoryakova E.A. ^1,3 , Tokmakova A.D. ^1,2,4 , Shpirt A.M. ⁵ , Kabilov M.R. ⁶ , Obraztsova E.A. ¹ , Shashkov A.S. ⁵ , Ignatov A.N. ^2,7 , Knirel Y.A. ⁵ , Dzhalilov Fevzi S.-U. ² , Miroshnikov K. ^1,2

Affiliations

1	Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
2	Department of Plant Protection, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, 127134 Moscow, Russia
3	Lomonosov Moscow State University
4	School of Biological and Medical Physics, Moscow Institute of Physics and Technology (MIPT), 141701 Dolgoprudny, Russia
5	N. D. Zelinsky Institute of Organic Chemistry, RAS
6	Institute of Chemical Biology and Fundamental Medicine SB RAS
7	Agrarian and Technological Institute, RUDN University, 117198 Moscow, Russia

Novel, closely related phages Possum and Horatius infect Pectobacterium versatile, a phytopathogen causing soft rot in potatoes and other essential plants. Their properties and genomic composition define them as N4-like bacteriophages of the genus Cbunavirus, a part of a recently formed family Schitoviridae. It is proposed that the adsorption apparatus of these phages consists of tail fibers connected to the virion through an adapter protein. Tail fibers possess an enzymatic domain. Phage Possum uses it to deacetylate O-polysaccharide on the surface of the host strain to provide viral attachment. Such an infection mechanism is supposed to be common for all Cbunavirus phages and this feature should be considered when designing cocktails for phage control of soft rot.

Lukianova A. , Evseev P.V. , Shneider M.M. , Dvoryakova E.A. , Tokmakova A.D. , Shpirt A.M. , Kabilov M.R. , Obraztsova E.A. , Shashkov A.S. , Ignatov A.N. , Knirel Y.A. , Dzhalilov F.S.-U. , Miroshnikov K.
Pectobacterium versatile Bacteriophage Possum: A Complex Polysaccharide-Deacetylating Tail Fiber as a Tool for Host Recognition in Pectobacterial Schitoviridae
International Journal of Molecular Sciences. 2022. V.23. N19. P.11043. DOI: 10.3390/ijms231911043 WOS Scopus OpenAlex

Published print:

Sep 22, 2022

Web of science:	WOS:000867778100001
Scopus:	2-s2.0-85139930266
OpenAlex:	W4296481846

DB	Citing
Scopus	5
OpenAlex	9
Web of science	6