Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase Научная публикация
| Журнал |
Data in Brief
ISSN: 2352-3409 |
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| Вых. Данные | Год: 2020, Том: 29, Номер статьи : 105188, Страниц : DOI: 10.1016/j.dib.2020.105188 | ||||
| Ключевые слова | Isothermal amplification, Multimerization, Bst exo- DNA polymerase, Phosphoryl guanidine oligonucleotides (PGO) | ||||
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Реферат:
Over the last two decades, isothermal amplification of nucleic acids has gained more attention due to a
number of advantages over the widely used polymerase chain reaction. For isothermal amplification,
DNA polymerases with strand-displacement activity are needed, and Bst exo- polymerase is one of the
most commonly used. Unfortunately, Bst exo- causes nonspecific DNA amplification (so-called multi-
merization) under isothermal conditions that results in undesirable products (multimers) consisting of
tandem nucleotide repeats. Multimerization occurs only for short ssDNA or primer dimers, and the ef-
ficiency of multimerization depends significantly on the reaction conditions, but slightly depends on the
sequence of DNA templates. In this study we report the prevention of DNA multimerization using a new
type of modified oligonucleotide primers with internucleosidic phosphates containing 1,3-dimethyl-2-
imino-imidazolidine moieties (phosphoryl guanidine (PG) groups). Primers with one, two or three PG
groups located at the 30- or 50-ends or in the middle of the primers were designed. It turned out, such
bulky groups interfere with the moving of Bst exo- polymerase along DNA chains. However, one modified
phosphate does not notably affect the efficiency of polymerization, and the elongation is completely
inhibited only when three contiguous modifications occur. Multimerization of the linear ssDNA tem-
plates is blocked by three modifications in the middle of both primers whereas specific amplification of
the circular ssDNA by rolling circle amplification is not inhibited. Thus, incorporation of three PG groups
is sufficient to prevent multimerization and allows to create improved primers for reliable isothermal
amplification with Bst exo- DNA polymerase.
Библиографическая ссылка:
Garafutdinov R.R.
, Sakhabutdinova A.R.
, Kupryushkin M.S.
, Pyshnyi D.V.
Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase
Data in Brief. 2020. V.29. 105188 . DOI: 10.1016/j.dib.2020.105188 WOS Scopus OpenAlex
Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase
Data in Brief. 2020. V.29. 105188 . DOI: 10.1016/j.dib.2020.105188 WOS Scopus OpenAlex
Идентификаторы БД:
| Web of science: | WOS:000529377400025 |
| Scopus: | 2-s2.0-85078875184 |
| OpenAlex: | W3002954822 |