Insights into Mechanisms of Damage Recognition and Catalysis by APE1-like Enzymes Full article
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International Journal of Molecular Sciences
, E-ISSN: 1422-0067 |
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| Output data | Year: 2022, Volume: 23, Number: 8, Article number : 4361, Pages count : DOI: 10.3390/ijms23084361 | ||||
| Tags | active-site plasticity; apurinic/apyrimidinic endonuclease; base excision repair; conformational dynamics; damaged nucleotide; nucleotide eversion; nucleotide incision activity | ||||
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Abstract:
Apurinic/apyrimidinic (AP) endonucleases are the key DNA repair enzymes in the base excision repair (BER) pathway, and are responsible for hydrolyzing phosphodiester bonds on the 5′ side of an AP site. The enzymes can recognize not only AP sites but also some types of damaged bases, such as 1,N6-ethenoadenosine, α-adenosine, and 5,6-dihydrouridine. Here, to elucidate the mechanism underlying such a broad substrate specificity as that of AP endonucleases, we per-formed a computational study of four homologous APE1-like endonucleases: insect (Drosophila melanogaster) Rrp1, amphibian (Xenopus laevis) APE1 (xAPE1), fish (Danio rerio) APE1 (zAPE1), and human APE1 (hAPE1). The contact between the amino acid residues of the active site of each ho-mologous APE1-like enzyme and the set of damaged DNA substrates was analyzed. A comparison of molecular dynamic simulation data with the known catalytic efficiency of these enzymes al-lowed us to gain a deep insight into the differences in the efficiency of the cleavage of various damaged nucleotides. The obtained data support that the amino acid residues within the “damage recognition” loop containing residues Asn222–Ala230 significantly affect the catalytic-complex formation. Moreover, every damaged nucleotide has its unique position and a specific set of interactions with the amino acid residues of the active site. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Cite:
Bulygin A.A.
, Fedorova O.S.
, Kuznetsov N.А.
Insights into Mechanisms of Damage Recognition and Catalysis by APE1-like Enzymes
International Journal of Molecular Sciences. 2022. V.23. N8. 4361 . DOI: 10.3390/ijms23084361 WOS Scopus РИНЦ OpenAlex
Insights into Mechanisms of Damage Recognition and Catalysis by APE1-like Enzymes
International Journal of Molecular Sciences. 2022. V.23. N8. 4361 . DOI: 10.3390/ijms23084361 WOS Scopus РИНЦ OpenAlex
Dates:
| Published print: | Apr 14, 2022 |
Identifiers:
| Web of science: | WOS:000787516800001 |
| Scopus: | 2-s2.0-85128152618 |
| Elibrary: | 48428150 |
| OpenAlex: | W4224107747 |