The Blood of the HIV-Infected Patients Contains kappa-IgG, lambda-IgG, and Bispecific kappa lambda-IgG, Which Possess DNase and Amylolytic Activity

The Blood of the HIV-Infected Patients Contains kappa-IgG, lambda-IgG, and Bispecific kappa lambda-IgG, Which Possess DNase and Amylolytic Activity Full article

Journal

Life
ISSN: 2075-1729

Output data

Year: 2022, Volume: 12, Number: 2, Article number : 304, Pages count : DOI: 10.3390/life12020304

Tags

HIV; bispecific antibodies; IgG; immunoglobulin G; catalytic activity; abzymes; DNAse activity; DNA hydrolysis; amylolytic activity; ELISA

Authors

Timofeeva Anna ¹ , Sedykh Sergey ^1,2 , Maksimenko Lada ³ , Sedykh Tatyana ¹ , Skudarnov Sergey ⁴ , Ostapova Tatyana ⁴ , Yaschenko Svetlana ⁴ , Gashnikova Natalya ³ , Nevinsky Georgy ^1,2

Affiliations

1	(Данные Web of science) RAS, SB, Inst Chem Biol & Fundamental Med, Novosibirsk 630090, Russia
2	(Данные Web of science) Novosibirsk State Univ, Fac Nat Sci, Novosibirsk 630090, Russia
3	(Данные Web of science) State Res Ctr Virol & Biotechnol Vector, Koltsov 630559, Russia
4	(Данные Web of science) Krasnoyarsk Reg Ctr Prevent & Control AIDS, Krasnoyarsk 660049, Russia

Though hundreds of thousands of papers are currently being published on HIV/AIDS, only tens of hundreds of them are devoted to the antibodies generated during the disease. Most of these papers discuss antibodies in HIV/AIDS as a diagnostic tool, and some articles describe neutralizing antibodies as a promising treatment. In this paper, we used affinity chromatography and ELISA to isolate natural IgG from the blood of 26 HIV-infected patients. IgG preparations were separated into the subfractions containing different types of light chains, and catalytic activities of subfractions were analyzed. Here, we show for the first time that the blood of HIV patients contains ~20% of bispecific kappa lambda-IgG, presented with all IgG subclasses. Analysis of DNA-hydrolyzing and amylolytic activity show that most IgG preparations and subfractions are catalytically active. Our results expand the possible biological functions of natural IgG in HIV infection.

Timofeeva A. , Sedykh S. , Maksimenko L. , Sedykh T. , Skudarnov S. , Ostapova T. , Yaschenko S. , Gashnikova N. , Nevinsky G.
The Blood of the HIV-Infected Patients Contains kappa-IgG, lambda-IgG, and Bispecific kappa lambda-IgG, Which Possess DNase and Amylolytic Activity
Life. 2022. V.12. N2. 304 . DOI: 10.3390/life12020304 WOS Scopus OpenAlex

Published print:

Feb 17, 2022

Web of science:	WOS:000764610700001
Scopus:	2-s2.0-85125074090
OpenAlex:	W4212861813

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Web of science	2
Scopus	2
OpenAlex	2