Cell Differentiation and Proliferation in the Bone Marrow and Other Organs of 2D2 Mice during Spontaneous Development of EAE Leading to the Production of Abzymes

Cell Differentiation and Proliferation in the Bone Marrow and Other Organs of 2D2 Mice during Spontaneous Development of EAE Leading to the Production of Abzymes Full article

Journal

Molecules
ISSN: 1420-3049

Output data

Year: 2022, Volume: 27, Number: 7, Article number : 2195, Pages count : DOI: 10.3390/molecules27072195

Tags

2D2; and C57BL/6 EAE mice; catalytic antibodies; development of experimental autoimmune encephalomyelitis (EAE); hematopoietic stem cells differentiation; lymphocyte proliferation in different organs; Th

Authors

Aulova K.S. ¹ , Urusov A.E. ¹ , Toporkova L.B. ² , Sedykh S.E. ¹ , Shevchenko J.A. ² , Tereshchenko V.P. ² , Sennikov S.V. ² , Orlovskaya I.A. ² , Nevinsky G.A. ¹

Affiliations

1	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russian Federation
2	Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russian Federation

The exact cellular and molecular mechanisms of multiple sclerosis and other autoimmune diseases have not been established. Autoimmune pathologies are known to be associated with faults in the immune system and changes in the differentiation profiles of bone marrow stem cells. This study analyzed various characteristics of experimental autoimmune encephalomyelitis (EAE) in 2D2 mice. Differentiation profiles of six hematopoietic stem cells of bone marrow were found to significantly differ in 2D2 male and female mice during the spontaneous development of EAE. In addition, we found various properties of B and T cells, CD4+ and CD8+ lymphocytes in blood and several organs (bone marrow, spleen, thymus, and lymph nodes) of 2D2 male and female mice to be considerably different. These changes in hematopoietic stem cells differentiation profiles and level of lymphocyte proliferation in various organs of 2D2 mice were found to induce the production of IgGs against DNA, myelin basic protein, and myelin oligodendrocyte glycopro-tein, increasing the number of autoantibodies hydrolyzing these substrates. We compared the changes of these immunological and biochemical parameters in 2D2 mice with those of mice of two other lines (Th and C57BL/6), also prone to spontaneous development of EAE. Some noticeable and even extreme variations were found in the time-related development of parameters between male and female mice of 2D2, Th, and C57BL/6 lines. Despite some differences, mice of all three lines demonstrated the changes in hematopoietic stem cells profiles, lymphocyte content, and production of catalytic autoantibodies. Given that these changes are harmful to mice, we believe them to cause the development of experimental autoimmune encephalomyelitis. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Aulova K.S. , Urusov A.E. , Toporkova L.B. , Sedykh S.E. , Shevchenko J.A. , Tereshchenko V.P. , Sennikov S.V. , Orlovskaya I.A. , Nevinsky G.A.
Cell Differentiation and Proliferation in the Bone Marrow and Other Organs of 2D2 Mice during Spontaneous Development of EAE Leading to the Production of Abzymes
Molecules. 2022. V.27. N7. 2195 . DOI: 10.3390/molecules27072195 WOS Scopus РИНЦ OpenAlex

Published print:

Mar 28, 2022

Web of science:	WOS:000780532200001
Scopus:	2-s2.0-85127886709
Elibrary:	48426608
OpenAlex:	W4220941086

DB	Citing
Scopus	2
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Web of science	4
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