Application of W-band F-19 electron nuclear double resonance (ENDOR) spectroscopy to distance measurement using a trityl spin probe and a fluorine label

Application of W-band F-19 electron nuclear double resonance (ENDOR) spectroscopy to distance measurement using a trityl spin probe and a fluorine label Full article

Journal

Physical Chemistry Chemical Physics
ISSN: 1463-9076

Output data

Year: 2022, Volume: 24, Number: 10, Pages: 5982-6001 Pages count : 20 DOI: 10.1039/d1cp05445g

Authors

Asanbaeva N.B. ¹ , Sukhanov A.A. ^2,3 , Diveikina A.A. ¹ , Rogozhnikova O.Y. ¹ , Trukhin D. ¹ , Tormyshev V.M. ¹ , Chubarov A.S. ⁴ , Maryasov A.G. ¹ , Genaev A.M. ¹ , Shernyukov A. ¹ , Salnikov G.E. ¹ , Lomzov A.A. ⁴ , Pyshnyi D. ⁴ , Bagryanskaya E.G. ¹

Affiliations

1	(Данные Web of science) NN Vorozhtsov Novosibirsk Inst Organ Chem SB RAS, 9 Pr Ak Lavrentjeva, Novosibirsk 630090, Russia
2	(Данные Web of science) RAS, Zavoisky Phys Tech Inst, FRC Kazan Sci Ctr, 10-7 Sibirsky Tract, Kazan 420029, Russia
3	(Данные Web of science) Kazan State Med Univ, Butlerova St 49, Kazan 420012, Russia
4	(Данные Web of science) Inst Chem Biol & Fundamental Med SB RAS, 8 Pr Ak Lavrentjeva, Novosibirsk 630090, Russia

Recently, Marina Bennati and coworkers (M. Bennati et al., Angew. Chem., Int. Ed., 2020, 59, 373-379., M. Bennati et al., J. Magn. Reson., 2021, 333, 107091) proposed to use electron nuclear double resonance (ENDOR) spectroscopy in the W-band for a pair of labels, nitroxide and F-19, for measurements of short (0.5-1.0 nm) distances in biomolecules. In our paper, we investigated the suitability of high-field ENDOR spectroscopy in the W-band for pairs of triarylmethyl and fluorine labels using five newly synthesized model compounds. It is shown that the application of strong magnetic fields allows distinguishing nuclear frequencies of F-19 and protons with sufficient resolution. On the one hand, in contrast to nitroxides, for triarylmethyl radicals, it is not necessary to obtain spectra in different orientations owing to low g-factor anisotropic and long electron spin relaxation times of triarylmethyls. On the other hand, the size of the triarylmethyl radical is substantially larger than that of nitroxide and comparable with measured distances. We theoretically analyzed the suitability of the dipole-dipole approach for triarylmethyl to be used in a F-19 ENDOR experiment and determined limitations of this approach. Finally, for comparison, we performed paramagnetic relaxation enhancement (PRE) NMR on the same compounds. In addition, we applied this approach to study the process of a thiol exchange between molecules of triarylmethyl-labeled and F-19-labeled human serum albumin (HSA).

Asanbaeva N.B. , Sukhanov A.A. , Diveikina A.A. , Rogozhnikova O.Y. , Trukhin D. , Tormyshev V.M. , Chubarov A.S. , Maryasov A.G. , Genaev A.M. , Shernyukov A. , Salnikov G.E. , Lomzov A.A. , Pyshnyi D. , Bagryanskaya E.G.
Application of W-band F-19 electron nuclear double resonance (ENDOR) spectroscopy to distance measurement using a trityl spin probe and a fluorine label
Physical Chemistry Chemical Physics. 2022. V.24. N10. P.5982-6001. DOI: 10.1039/d1cp05445g WOS Scopus OpenAlex

Published print:

Feb 18, 2022

Web of science:	WOS:000760363100001
Scopus:	2-s2.0-85126072697
OpenAlex:	W4213294350

DB	Citing
Web of science	14
Scopus	12
OpenAlex	14