Abstract: Photoregulated systems are interesting and prospective instruments to affect gene expression. An approach to the synthesis of photoregulated siRNA has been proposed for RNA interference activated by light irradiation. For temporary protection, a bulky group has been introduced in siRNA through the photocleavable linker. We have used amino-modified cholesterol and trileucine as bulky ligands, which have been attached to siRNA by the interaction with the hydroxyl group activated with disuccinimidyl carbonate. The efficient UV-induced removal of the ligands introduced through the photocleavable linker has been demonstrated. Using the proposed approach, siRNA molecules bearing cholesterol residues at the 5'-terminus of sense- and/or antisense strands have been synthesized. They were targeted to mRNA of EGFP (enhanced green fluorescent protein). The photoinduced inhibition of the EGFP gene expression in the EGFP-expressing HEK293FT cells has been shown to be possible. © 2022, Pleiades Publishing, Ltd.

Cite: Akhmetova E.A. , Kim D.V. , Dome A.S. , Meschaninova M.I. , Novopashina D.S.
Photocaged Small Interfering RNA
Russian Journal of Bioorganic Chemistry. 2022. V.48. N5. P.1036 - 1042. DOI: 10.1134/S106816202205003X WOS Scopus РИНЦ OpenAlex

Original: Ахметова Е.А. , Ким Д.В. , Доме А.С. , Мещанинова М.И. , Новопашина Д.С.
Новый подход к синтезу фотоблокированных малых интерферирующих РНК для активируемой светом РНК-интерференции
Биоорганическая химия. 2022. Т.48. №5. С.580-588. DOI: 10.31857/S0132342322050037 РИНЦ OpenAlex

Identifiers:

Web of science:	WOS:000857776400015
Scopus:	2-s2.0-85138718618
Elibrary:	51544634
OpenAlex:	W4296995257

Citing: Пока нет цитирований

Altmetrics: