Transcriptome Changes in Glioma Cells Cultivated under Conditions of Neurosphere Formation

Transcriptome Changes in Glioma Cells Cultivated under Conditions of Neurosphere Formation Full article

Journal

Cells
ISSN: 2073-4409

Output data

Year: 2022, Volume: 11, Number: 19, Article number : 3106, Pages count : DOI: 10.3390/cells11193106

Tags

cancer stem cells; epithelial to mesenchymal transition; glioblastoma; glioma; neurospheres; pro-neural to mesenchymal transition

Authors

Vasileva N.S. ¹ , Kuligina E.V. ¹ , Dymova M.A. ¹ , Savinovskaya Y.I. ¹ , Zinchenko N.D. ¹ , Ageenko A.B. ¹ , Mishinov S.V. ² , Dome A.S. ¹ , Stepanov G.A. ¹ , Richter V.A. ¹ , Semenov D.V. ¹

Affiliations

1	Institute of Chemical Biology and Fundamental Medicine, Russian Academy of Sciences, Siberian Branch, Lavrentyev Avenue 8, Novosibirsk, 630090, Russian Federation
2	Novosibirsk Research Institute of Traumatology and Orthopedics n.a. Ya.L. Tsivyan, Department of Neurosurgery, Frunze Street 17, Novosibirsk, 630091, Russian Federation

Glioma is the most common and heterogeneous primary brain tumor. The development of a new relevant preclinical models is necessary. As research moves from cultures of adherent gliomas to a more relevant model, neurospheres, it is necessary to understand the changes that cells undergo at the transcriptome level. In the present work, we used three patient-derived gliomas and two immortalized glioblastomas, while their cultivation was carried out under adherent culture and neurosphere (NS) conditions. When comparing the transcriptomes of monolayer (ML) and NS cell cultures, we used Enrichr genes sets enrichment analysis to describe transcription factors (TFs) and the pathways involved in the formation of glioma NS. It was observed that NS formation is accompanied by the activation of five common gliomas of TFs, SOX2, UBTF, NFE2L2, TCF3 and STAT3. The sets of transcripts controlled by TFs MYC and MAX were suppressed in NS. Upregulated genes are involved in the processes of the epithelial–mesenchymal transition, cancer stemness, invasion and migration of glioma cells. However, MYC/MAX-dependent downregulated genes are involved in translation, focal adhesion and apical junction. Furthermore, we found three EGFR and FGFR signaling feedback regulators common to all analyzed gliomas—SPRY4, ERRFI1, and RAB31—which can be used for creating new therapeutic strategies of suppressing the invasion and progression of gliomas. © 2022 by the authors.

Vasileva N.S. , Kuligina E.V. , Dymova M.A. , Savinovskaya Y.I. , Zinchenko N.D. , Ageenko A.B. , Mishinov S.V. , Dome A.S. , Stepanov G.A. , Richter V.A. , Semenov D.V.
Transcriptome Changes in Glioma Cells Cultivated under Conditions of Neurosphere Formation
Cells. 2022. V.11. N19. 3106 . DOI: 10.3390/cells11193106 WOS Scopus РИНЦ OpenAlex

Published print:

Oct 2, 2022

Web of science:	WOS:000866671800001
Scopus:	2-s2.0-85139788962
Elibrary:	49589522
OpenAlex:	W4303628714

DB	Citing
Scopus	7
Web of science	7
OpenAlex	8