A Mechanocomposite Based on Biogenic Silica and Green Tea Flavonoids Modulates Adaptability of Strawberry Microclones to In Vitro and Ex Vitro Conditions Full article
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Journal of Soil Science and Plant Nutrition
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| Output data | Year: 2023, Volume: 23, Pages: 612-627 Pages count : 16 DOI: 10.1007/s42729-022-01069-3 | ||||||
| Tags | Strawberry · Micropropagation · Oxidative stress · Biogenic silica · Antioxidant defense system · Phytohormone | ||||||
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Abstract:
The purpose was to assess efects of a mechanocomposite (MC) based on biogenic silica and green tea favonoids in terms
of alleviation of the impact of abiotic stressors on strawberry plantlets at diferent stages of clonal micropropagation. An
experiment was set up with or without MC treatment at multiplication, in vitro rooting, and ex vitro acclimation stages. We
quantifed hydrogen peroxide (H2O2) as a marker of oxidative stress along with antioxidants (non- and enzymatic), phytohormones, and biomass parameters. At the multiplication stage, MC treatment increased the level of endogenous H2O2 (2.3-
fold), the activity of antioxidant enzymes (superoxide dismutase, catalase, and peroxidase), and the total phenolic content.
Nonetheless, the MC decreased levels of phytohormones (indole-3-acetic acid, trans-zeatin, and abscisic acid). The addition
of MC at the in vitro rooting stage lowered the H2O2 content 1.6-fold, while the activity of antioxidant enzymes signifcantly
increased, compared to the MC-free group; in the MC group, the studied phytohormones showed maximum values. Efects
of the MC on phenolic compounds varied. Concentrations of some compounds signifcantly increased, while the majority of
these compounds showed a decline. At the ex vitro acclimation stage, MC treatment raised the level of H2O2 in microshoots
in comparison to in vitro stages (this efect was attributed to the enhancement of antioxidant enzymes’ activities), accompanied by downregulation of the phytohormones and upregulation of most of phenolic compounds. The MC-induced efects
on growth and development were confrmed by greater shoot biomass. During micropropagation, MC application allows
plants to overcome oxidative stress through modulation of activities of antioxidant enzymes and phytohormone levels and
a realignment of the profle of phenolic compounds
Cite:
Ambros E.
, Karpova E.
, Kotsupiy O.
, Trofimova E.
, Zakabkuk G.A.
, Chernonosov A.A.
, Koval V.V.
, Novikova T.
A Mechanocomposite Based on Biogenic Silica and Green Tea Flavonoids Modulates Adaptability of Strawberry Microclones to In Vitro and Ex Vitro Conditions
Journal of Soil Science and Plant Nutrition. 2023. V.23. P.612-627. DOI: 10.1007/s42729-022-01069-3 WOS Scopus РИНЦ OpenAlex
A Mechanocomposite Based on Biogenic Silica and Green Tea Flavonoids Modulates Adaptability of Strawberry Microclones to In Vitro and Ex Vitro Conditions
Journal of Soil Science and Plant Nutrition. 2023. V.23. P.612-627. DOI: 10.1007/s42729-022-01069-3 WOS Scopus РИНЦ OpenAlex
Dates:
| Published online: | Nov 28, 2022 |
Identifiers:
| Web of science: | WOS:000889388400005 |
| Scopus: | 2-s2.0-85142826484 |
| Elibrary: | 54475057 |
| OpenAlex: | W4310135766 |