Milk Exosomes: Next-Generation Agents for Delivery of Anticancer Drugs and Therapeutic Nucleic Acids Full article
| Journal |
International Journal of Molecular Sciences
, E-ISSN: 1422-0067 |
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| Output data | Year: 2023, Volume: 24, Article number : 10194, Pages count : DOI: 10.3390/ijms241210194 | ||||
| Tags | exosomes; milk exosomes; milk vesicles; extracellular vesicles; drug delivery; target delivery; visualization; cargo loading | ||||
| Authors |
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| Affiliations |
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Funding (2)
| 1 | МИНИСТЕРСТВО НАУКИ И ВЫСШЕГО ОБРАЗОВАНИЯ РОССИЙСКОЙ ФЕДЕРАЦИИ | ПФНИ РФ (2021-2030) 0245-2021-0009 |
| 2 | Russian Science Foundation | РНФ_мол_гр №18-74-10055 |
Abstract:
Exosomes are nanovesicles 40–120 nm in diameter secreted by almost all cell types and
providing humoral intercellular interactions. Given the natural origin and high biocompatibility,
the potential for loading various anticancer molecules and therapeutic nucleic acids inside, and the
surface modification possibility for targeted delivery, exosomes are considered to be a promising
means of delivery to cell cultures and experimental animal organisms. Milk is a unique natural source
of exosomes available in semi-preparative and preparative quantities. Milk exosomes are highly
resistant to the harsh conditions of the gastrointestinal tract. In vitro studies have demonstrated that
milk exosomes have an affinity to epithelial cells, are digested by cells by endocytosis mechanism,
and can be used for oral delivery. With milk exosome membranes containing hydrophilic and
hydrophobic components, exosomes can be loaded with hydrophilic and lipophilic drugs. This
review covers a number of scalable protocols for isolating and purifying exosomes from human, cow,
and horse milk. Additionally, it considers passive and active methods for drug loading into exosomes,
as well as methods for modifying and functionalizing the surface of milk exosomes with specific
molecules for more efficient and specific delivery to target cells. In addition, the review considers
various approaches to visualize exosomes and determine cellular localization and bio-distribution
of loaded drug molecules in tissues. In conclusion, we outline new challenges for studying milk
exosomes, a new generation of targeted delivery agents.
Cite:
Timofeeva A.M.
, Paramonik A.P.
, Sedykh S.E.
, Nevinsky G.A.
Milk Exosomes: Next-Generation Agents for Delivery of Anticancer Drugs and Therapeutic Nucleic Acids
International Journal of Molecular Sciences. 2023. V.24. 10194 . DOI: 10.3390/ijms241210194 WOS Scopus PMID OpenAlex
Milk Exosomes: Next-Generation Agents for Delivery of Anticancer Drugs and Therapeutic Nucleic Acids
International Journal of Molecular Sciences. 2023. V.24. 10194 . DOI: 10.3390/ijms241210194 WOS Scopus PMID OpenAlex
Dates:
| Published print: | Jun 15, 2023 |
Identifiers:
| Web of science: | WOS:001017157300001 |
| Scopus: | 2-s2.0-85164034176 |
| PMID: | 37373342 |
| OpenAlex: | W4380992275 |