Transcriptome Changes in Glioma Cells upon Infection with the Oncolytic Virus VV-GMCSF-Lact Научная публикация
Журнал |
Cells
ISSN: 2073-4409 |
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Вых. Данные | Год: 2023, Том: 12, Номер статьи : 2616, Страниц : DOI: 10.3390/cells12222616 | ||||||
Ключевые слова | glioma; glioblastoma; patient-derived cell cultures; virotherapy; vaccinia virus; oncolytic virus; VV-GMCSF-Lact; next-generation sequencing; transcriptome; differentially expressed genes | ||||||
Авторы |
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Организации |
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Информация о финансировании (2)
1 | МИНИСТЕРСТВО НАУКИ И ВЫСШЕГО ОБРАЗОВАНИЯ РОССИЙСКОЙ ФЕДЕРАЦИИ | ПФНИ РФ (2021-2030) 0245-2021-0005 |
2 | Российский научный фонд | РНФ №21-14-00195 |
Реферат:
Oncolytic virotherapy is a rapidly evolving approach that aims to selectively kill cancer cells.
We designed a promising recombinant vaccinia virus, VV-GMCSF-Lact, for the treatment of solid
tumors, including glioma. We assessed how VV-GMCSF-Lact affects human cells using immortalized
and patient-derived glioma cultures and a non-malignant brain cell culture. Studying transcriptome
changes in cells 12 h or 24 h after VV-GMCSF-Lact infection, we detected the common activation of
histone genes. Additionally, genes associated with the interferon-gamma response, NF-kappa B signaling
pathway, and inflammation mediated by chemokine and cytokine signaling pathways showed
increased expression. By contrast, genes involved in cell cycle progression, including spindle organization,
sister chromatid segregation, and the G2/M checkpoint, were downregulated following virus
infection. The upregulation of genes responsible for Golgi vesicles, protein transport, and secretion
correlated with reduced sensitivity to the cytotoxic effect of VV-GMCSF-Lact. Higher expression of
genes encoding proteins, which participate in the maturation of pol II nuclear transcripts and mRNA
splicing, was associated with an increased sensitivity to viral cytotoxicity. Genes whose expression
correlates with the sensitivity of cells to the virus are important for increasing the effectiveness of
cancer virotherapy. Overall, the results highlight molecular markers, biological pathways, and gene
networks influencing the response of glioma cells to VV-GMCSF-Lact.
Библиографическая ссылка:
Semenov D.V.
, Vasileva N.S.
, Dymova M.A.
, Mishinov S.V.
, Savinovskaya Y.I.
, Ageenko A.B.
, Dome A.S.
, Zinchenko N.D.
, Stepanov G.A.
, Kochneva G.V.
, Richter V.A.
, Kuligina E.V.
Transcriptome Changes in Glioma Cells upon Infection with the Oncolytic Virus VV-GMCSF-Lact
Cells. 2023. V.12. 2616 . DOI: 10.3390/cells12222616 WOS Scopus РИНЦ
Transcriptome Changes in Glioma Cells upon Infection with the Oncolytic Virus VV-GMCSF-Lact
Cells. 2023. V.12. 2616 . DOI: 10.3390/cells12222616 WOS Scopus РИНЦ
Даты:
Опубликована в печати: | 12 нояб. 2023 г. |
Идентификаторы БД:
Web of science | WOS:001119784000001 |
Scopus | 2-s2.0-85177830868 |
РИНЦ | 55061302 |
PMID (PubMed) | 7998351 |
OpenAlex | W4388635082 |
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