Response of PRIMPOL-Knockout Human Lung Adenocarcinoma A549 Cells to Genotoxic Stress Обзор
Журнал |
Biochemistry (Moscow)
ISSN: 0006-2979 |
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Вых. Данные | Год: 2023, Том: 88, Номер: 11, Страницы: 1933-1943 Страниц : 11 DOI: 10.1134/s0006297923110214 | ||||||||||||
Ключевые слова | DNA damage, damage tolerance, apoptosis, PRIMPOL, primase, knockout cell lines | ||||||||||||
Авторы |
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Организации |
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Реферат:
Human DNA primase/polymerase PrimPol synthesizes DNA primers de novo after replication fork stalling at the sites of DNA damage, thus contributing to the DNA damage tolerance. The role of PrimPol in response to the different types of DNA damage is poorly understood. We knocked out the PRIMPOL gene in the lung carcinoma A549 cell line and characterized the response of the obtained cells to the DNA damage caused by hydrogen peroxide, methyl methanesulfonate (MMS), cisplatin, bleomycin, and ionizing radiation. The PRIMPOL knockout reduced the number of proliferating cells and cells in the G2 phase after treatment with MMS and caused a more pronounced delay of the S phase in the cisplatin-treated cells. Ionizing radiation at a dose of 10 Gy significantly increased the content of apoptotic cells among the PRIMPOL-deficient cells, while the proportion of cells undergoing necroptosis increased in both parental and knockout cells at any radiation dose. The viability of PRIMPOL-deficient cells upon the hydrogen peroxide-induced oxidative stress increased compared to the control cells, as determined by the methyl tetrazolium (MTT) assay. The obtained data indicate the involvement of PRIMPOL in the modulation of adaptive cell response to various types of genotoxic stress.
Библиографическая ссылка:
Gromova A.S.
, Boldinova E.O.
, Kim D.V.
, Chuprov-Netochin R.N.
, Leonov S.V.
, Pustovalova M.V.
, Zharkov D.O.
, Makarova A.V.
Response of PRIMPOL-Knockout Human Lung Adenocarcinoma A549 Cells to Genotoxic Stress
Biochemistry (Moscow). 2023. V.88. N11. P.1933-1943. DOI: 10.1134/s0006297923110214 WOS Scopus
Response of PRIMPOL-Knockout Human Lung Adenocarcinoma A549 Cells to Genotoxic Stress
Biochemistry (Moscow). 2023. V.88. N11. P.1933-1943. DOI: 10.1134/s0006297923110214 WOS Scopus
Оригинальная:
Громова А.С.
, Болдинова Е.О.
, Ким Д.В.
, Чупров-Неточин Р.Н.
, Леонов С.В.
, Пустовалова М.В.
, Жарков Д.О.
, Макарова А.В.
Особенности ответа клеток карциномы легких человека A549 с нокаутированным геном PRIMPOL на генотоксический стресс
БИОХИМИЯ. 2023. Т.88. №11. С.2340-2352. DOI: 10.31857/S0320972523110222 РИНЦ
Особенности ответа клеток карциномы легких человека A549 с нокаутированным геном PRIMPOL на генотоксический стресс
БИОХИМИЯ. 2023. Т.88. №11. С.2340-2352. DOI: 10.31857/S0320972523110222 РИНЦ
Даты:
Принята к публикации: | 15 сент. 2023 г. |
Идентификаторы БД:
Web of science | WOS:001110730800022 |
Scopus | 2-s2.0-85178198231 |
OpenAlex | W4389165980 |
Цитирование в БД:
Пока нет цитирований