Identification, Characterization, and Genome Analysis of Two Novel Temperate Pseudomonas protegens phages PseuP_222 and PseuP_224 Научная публикация
Журнал |
Microorganisms
ISSN: 2076-2607 |
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Вых. Данные | Год: 2023, Том: 11, Номер: 6, Номер статьи : 1456, Страниц : DOI: 10.3390/microorganisms11061456 | ||||
Ключевые слова | Pseudomonas protegens; environmental Pseudomonas phage; lambdoid phage; comparative genomics; proteomic analysis | ||||
Авторы |
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Организации |
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Реферат:
Two novel P. protegens bacteriophages PseuP_222 and Pseu_224 and their host P. protegens
CEMTC 4060 were isolated from the same sample (Inya river, Siberia). Both phages have siphovirus
morphology and belong to lambdoid phages. Comparative genome analysis revealed a low nucleotide
and amino acid sequence similarity of PseuP_222 and PseuP_224 between themselves, and between
them and other lambdoid phages. Bioinformatics analysis indicated that PseuP_222 and PseuP_224
are members of a genetically diverse group of phages of environmental Pseudomonas spp.; this group
is distant from a large group of P. aeruginosa phages. In phylogenetic trees, the positioning of the
terminase large subunits, major capsid proteins, tail tape measure proteins, and CI-like repressors of
PseuP_222 and PseuP_224 were remote and changed relative to those of the Escherichia lambda phage
and lambdoid phages of Pseudomonas spp. However, the nucleoid-associated protein NdpA/YejK and
P5-like structural protein from both phages showed high similarity and were not found in lambda
phage and other lambdoid phages of Pseudomonas spp. Substantial divergences of the PseuP_222
and PseuP_224 genomes and proteomes indicated that the evolutionary history of these phages was
mostly independent and they probably began to use one host only recently.
Библиографическая ссылка:
Morozova V.V.
, Kozlova Y.N.
, Tikunov A.Y.
, Babkin I.V.
, Ushakova T.A.
, Bardasheva A.V.
, Jdeed G.
, Zhirakovskaia E.V.
, Mogileva A.
, Netesov S.
, Tikunova N.V.
Identification, Characterization, and Genome Analysis of Two Novel Temperate Pseudomonas protegens phages PseuP_222 and PseuP_224
Microorganisms. 2023. Т.11. №6. 1456 . DOI: 10.3390/microorganisms11061456 WOS Scopus
Identification, Characterization, and Genome Analysis of Two Novel Temperate Pseudomonas protegens phages PseuP_222 and PseuP_224
Microorganisms. 2023. Т.11. №6. 1456 . DOI: 10.3390/microorganisms11061456 WOS Scopus
Даты:
Опубликована в печати: | 31 мая 2023 г. |
Идентификаторы БД:
Web of science | WOS:001014501500001 |
Scopus | 2-s2.0-85163813917 |
PMID (PubMed) | 37374958 |
OpenAlex | W4378983160 |