Non-Targeted Screening of Metabolites in Aqueous-Ethanol Extract from Spiraea hypericifolia (Rosaceae) Using LC-HRMS

Non-Targeted Screening of Metabolites in Aqueous-Ethanol Extract from Spiraea hypericifolia (Rosaceae) Using LC-HRMS Full article

Journal

International Journal of Molecular Sciences
, E-ISSN: 1422-0067

Output data

Year: 2023, Volume: 24, Article number : 13872, Pages count : 15 DOI: 10.3390/ijms241813872

Tags

Spiraea hypericifolia; LC-HRMS; HPLC; metabolome; flavonoid; antioxidant activity

Authors

Kostikova V.A. ¹ , Petrova N.V. ² , Shaldaeva T.M. ¹ , Koval V.V. ³ , Chernonosov A.A. ³

Affiliations

1	Central Siberian Botanical Garden, Siberian Branch, Russian Academy of Sciences (SB RAS),Novosibirsk 630090, Russia
2	Komarov Botanical Institute, Russian Academy of Sciences, St. Petersburg 197022, Russia
3	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences (SB RAS), Novosibirsk 630090, Russia

By means of liquid chromatography combined with high-resolution mass spectrometry, metabolite profiling was performed on an aqueous-ethanol extract from Spiraea hypericifolia (Rosaceae) collected in Siberia (Russia). Up to 140 compounds were found in the extract, of which 47 were tentatively identified. The identified compounds were amino acids, sugars, phenylpropanoids, fatty acids and their derivatives, triterpenoids, flavonoids, and others. A quantitative analysis showed the predominance of phenolcarboxylic acids and flavonoids in the studied extract, but a qualitative analysis revealed the higher structural diversity of flavonoids. Of the 23 identified flavonoids, 13 were flavonols: quercetin, hyperoside, isoquercitrin, reynoutrin, avicularin, rutin, quercetin-3-O-(6″-O-malonyl)-β-D-glucoside, 3-O-methylquercetin-3′-O-β-D-glucopyranoside, isorhamnetin, rhamnetin-3-O-β-D-xylopyranosyl-β-D-glucopyranoside, kaempferol, tiliroside, and trifolin; six were catechins: catechin, (−)-epicatechin, (+)-epicatechin, (+)-catechin-7-O-β-D-xyloside, (2S,3R)-3,5-dihydroxy-2-(4-hydroxyphenyl)-3,4-dihydro-2H-chromen-7-yl-β-D-glucopyranoside, and catechin 7-O-apiofuranoside; two are isoflavones: genistin and genistein; and one was a flavone (luteolin-4′-O-β-D-glucopyranoside) and another was an anthocyanidin (pelargonidin). The aqueous-ethanol extract from S. hypericifolia showed antioxidant activity (half-maximal inhibitory concentration 102.95 μg/mL), which was likely related to the high concentrations of phenolcarboxylic acids (229.6 mg/g), flavonoids (118.3 mg/g), and tannins (62.9 mg/g).

Kostikova V.A. , Petrova N.V. , Shaldaeva T.M. , Koval V.V. , Chernonosov A.A.
Non-Targeted Screening of Metabolites in Aqueous-Ethanol Extract from Spiraea hypericifolia (Rosaceae) Using LC-HRMS
International Journal of Molecular Sciences. 2023. V.24. 13872 :1-15. DOI: 10.3390/ijms241813872 WOS Scopus OpenAlex

Published print:

Sep 4, 2023

Web of science:	WOS:001073770900001
Scopus:	2-s2.0-85172810395
OpenAlex:	W4386602541

DB	Citing
Web of science	4
OpenAlex	6
Scopus	2