Novel lipid‐oligonucleotide conjugates containing long‐chain sulfonyl phosphoramidate groups: Synthesis and biological properties

Novel lipid‐oligonucleotide conjugates containing long‐chain sulfonyl phosphoramidate groups: Synthesis and biological properties Научная публикация

Журнал

Applied Sciences (Switzerland)
ISSN: 2076-3417

Вых. Данные

Год: 2021, Том: 11, Номер: 3, Номер статьи : 1174, Страниц : 15 DOI: 10.3390/app11031174

Ключевые слова

Cellular uptake; Cytotoxicity; Drug delivery; Macrophages; Multiple sclerosis; Nanoparticles; Repulsive guidance molecule a; Small interfering RNA; Therapeutic nucleic acid

Авторы

Derzhalova A. ^1,2 , Markov O. ² , Fokina A. ^1,3 , Shiohama Y. ⁴ , Zatsepin T. ^5,6 , Fujii M. ⁷ , Zenkova M. ² , Stetsenko D. ^1,3

Организации

1	Department of Physics, Novosibirsk State University, Novosibirsk, 630090, Russian Federation
2	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russian Federation
3	Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 63090, Russian Federation
4	Tropical Biosphere Research Center, University of the Ryukyus, Okinawa, 903‐0213, Japan
5	Skolkovo Institute of Science and Technology, Innovation Centre Skolkovo, Moscow, 121205, Russian Federation
6	Department of Chemistry, Lomonosov Moscow State University, Moscow, 119991, Russian Federation
7	Department of Biological & Environmental Chemistry, Kindai University, Fukuoka, Iizuka, 820‐8555, Japan

New lipid conjugates of DNA and RNA incorporating one to four [(4‐dodecylphenyl)sulfonyl]phosphoramidate or (hexadecylsulfonyl)phosphoramidate groups at internucleotidic positions near the 3′ or 5′‐end were synthesized and characterized. Low cytotoxicity of the conjugates and their ability to be taken up into cells without transfection agents were demonstrated. Lipidconjugated siRNAs targeting repulsive guidance molecules a (RGMa) have shown a comparable gene silencing activity in PK‐59 cells to unmodified control siRNA when delivered into the cells via Lipofectamine mediated transfection. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Derzhalova A. , Markov O. , Fokina A. , Shiohama Y. , Zatsepin T. , Fujii M. , Zenkova M. , Stetsenko D.
Novel lipid‐oligonucleotide conjugates containing long‐chain sulfonyl phosphoramidate groups: Synthesis and biological properties
Applied Sciences (Switzerland). 2021. V.11. N3. 1174 :1-15. DOI: 10.3390/app11031174 WOS Scopus РИНЦ OpenAlex

Опубликована в печати:

27 янв. 2021 г.

Web of science:	WOS:000615074500001
Scopus:	2-s2.0-85100114976
РИНЦ:	44974555
OpenAlex:	W3125963021

БД	Цитирований
Scopus	10
РИНЦ	10
Web of science	9
OpenAlex	13