Novel lipid‐oligonucleotide conjugates containing long‐chain sulfonyl phosphoramidate groups: Synthesis and biological properties Full article
Journal |
Applied Sciences (Switzerland)
ISSN: 2076-3417 |
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Output data | Year: 2021, Volume: 11, Number: 3, Article number : 1174, Pages count : 15 DOI: 10.3390/app11031174 | ||||||||||||||
Tags | Cellular uptake; Cytotoxicity; Drug delivery; Macrophages; Multiple sclerosis; Nanoparticles; Repulsive guidance molecule a; Small interfering RNA; Therapeutic nucleic acid | ||||||||||||||
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Abstract:
New lipid conjugates of DNA and RNA incorporating one to four [(4‐dodecylphenyl)sulfonyl]phosphoramidate or (hexadecylsulfonyl)phosphoramidate groups at internucleotidic positions near the 3′ or 5′‐end were synthesized and characterized. Low cytotoxicity of the conjugates and their ability to be taken up into cells without transfection agents were demonstrated. Lipidconjugated siRNAs targeting repulsive guidance molecules a (RGMa) have shown a comparable gene silencing activity in PK‐59 cells to unmodified control siRNA when delivered into the cells via Lipofectamine mediated transfection. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Cite:
Derzhalova A.
, Markov O.
, Fokina A.
, Shiohama Y.
, Zatsepin T.
, Fujii M.
, Zenkova M.
, Stetsenko D.
Novel lipid‐oligonucleotide conjugates containing long‐chain sulfonyl phosphoramidate groups: Synthesis and biological properties
Applied Sciences (Switzerland). 2021. V.11. N3. 1174 :1-15. DOI: 10.3390/app11031174 WOS Scopus РИНЦ OpenAlex
Novel lipid‐oligonucleotide conjugates containing long‐chain sulfonyl phosphoramidate groups: Synthesis and biological properties
Applied Sciences (Switzerland). 2021. V.11. N3. 1174 :1-15. DOI: 10.3390/app11031174 WOS Scopus РИНЦ OpenAlex
Dates:
Published print: | Jan 27, 2021 |
Identifiers:
Web of science: | WOS:000615074500001 |
Scopus: | 2-s2.0-85100114976 |
Elibrary: | 44974555 |
OpenAlex: | W3125963021 |