Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples Научная публикация
| Журнал |
Magnetochemistry
ISSN: 2312-7481 |
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| Вых. Данные | Год: 2022, Том: 8, Номер: 8, Номер статьи : 85, Страниц : DOI: 10.3390/magnetochemistry8080085 | ||
| Ключевые слова | coating; functionalization; iron oxide nanoparticles; magnetic separation; nanomedicine; nucleic acid detection; nucleic acid extraction; nylon; polymer nanocomposites | ||
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| Организации |
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Реферат:
Magnetic Fe3O4 nanoparticles (MNPs) have great potential for nucleic acid separation, detection, and delivery. MNPs are considered a valuable tool in biomedicine due to their cost-effectiveness, stability, easy surface functionalization, and the possibility of the manipulations under a magnetic field. Herein, the synthesis of magnetic nylon 6 nanocomposites (MNPs@Ny6) was investigated. Transmission electron microscopy (TEM) was used for morphology and size analysis. A new method of UV-induced immobilization of oligonucleotides on MNPs@Ny6 for nucleic acid magnetic separation was proposed. MNPs@Ny6 shows a high oligonucleotide binding capacity of 2.2 nmol/mg with 73.3% loading efficiency. The proposed system has been applied to analyze model mixtures of target RNA on the total yeast RNA background. The RNA target isolation efficiency was 60% with high specificity. The bind RNA release was 88.8% in a quantity of 0.16 nmol/mg. The total RNA capture efficiency was 53%. Considering this, the MNPs@Ny6 is an attractive candidate for nucleic acids-specific magnetic isolation. © 2022 by the authors.
Библиографическая ссылка:
Bulgakova A.
, Chubarov A.
, Dmitrienko E.
Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples
Magnetochemistry. 2022. V.8. N8. 85 . DOI: 10.3390/magnetochemistry8080085 WOS Scopus OpenAlex
Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples
Magnetochemistry. 2022. V.8. N8. 85 . DOI: 10.3390/magnetochemistry8080085 WOS Scopus OpenAlex
Даты:
| Опубликована в печати: | 3 авг. 2022 г. |
Идентификаторы БД:
| Web of science: | WOS:000845737500001 |
| Scopus: | 2-s2.0-85136957694 |
| OpenAlex: | W4289711604 |