Abstract: Magnetic Fe3O4 nanoparticles (MNPs) have great potential for nucleic acid separation, detection, and delivery. MNPs are considered a valuable tool in biomedicine due to their cost-effectiveness, stability, easy surface functionalization, and the possibility of the manipulations under a magnetic field. Herein, the synthesis of magnetic nylon 6 nanocomposites (MNPs@Ny6) was investigated. Transmission electron microscopy (TEM) was used for morphology and size analysis. A new method of UV-induced immobilization of oligonucleotides on MNPs@Ny6 for nucleic acid magnetic separation was proposed. MNPs@Ny6 shows a high oligonucleotide binding capacity of 2.2 nmol/mg with 73.3% loading efficiency. The proposed system has been applied to analyze model mixtures of target RNA on the total yeast RNA background. The RNA target isolation efficiency was 60% with high specificity. The bind RNA release was 88.8% in a quantity of 0.16 nmol/mg. The total RNA capture efficiency was 53%. Considering this, the MNPs@Ny6 is an attractive candidate for nucleic acids-specific magnetic isolation. © 2022 by the authors.

Cite: Bulgakova A. , Chubarov A. , Dmitrienko E.
Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples
Magnetochemistry. 2022. V.8. N8. 85 . DOI: 10.3390/magnetochemistry8080085 WOS Scopus OpenAlex

Dates:

Published print:

Aug 3, 2022

Identifiers:

Web of science:	WOS:000845737500001
Scopus:	2-s2.0-85136957694
OpenAlex:	W4289711604

Citing:

DB	Citing
Scopus	8
Web of science	8
OpenAlex	11

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